Department of Cell Biology, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, Granada 18071, Spain.
Chem Senses. 2010 Feb;35(2):147-56. doi: 10.1093/chemse/bjp091. Epub 2009 Dec 23.
In acipenserids, crypt cells (CCs) have only been observed in juvenile specimens, and it has not been clarified whether they differentiate along with olfactory receptor neurons (ORNs) during the lecithotrophic stage or during later development stages. Furthermore, no detailed optical microscopy (OM) or electron microscopy study on the development of CCs has been published to date. In the present study, we used OM and electron microscopy to follow the development of CCs in Acipenser naccarii from hatching to the establishment of exogenous feeding. Based on these observations, we can affirm that CCs are present from the first few posthatching (PH) days. CCs appear with their nucleus close to the basal lamina of the epithelium and enveloped by supporting cells. In addition, from the beginning of day 2 PH, we observed cells with highly similar characteristics to those of CCs (absence of knob, abundant mitochondria and filamentous material in apical cytoplasm, numerous microtubules, and envelopment by supporting cells) but with cilia still remaining on their noninvaginated apical surface. We conclude that these cells may correspond to immature CCs in which the crypt, the final feature of their morphological differentiation, has not yet formed.
在鲟形目中，仅在幼年标本中观察到隐窝细胞 (CCs)，目前尚不清楚它们是在卵黄营养阶段还是在后期发育阶段与嗅觉受体神经元 (ORNs) 一起分化的。此外，迄今为止，尚未有关于 CCs 发育的详细光学显微镜 (OM) 或电子显微镜研究发表。在本研究中，我们使用 OM 和电子显微镜来跟踪从孵化到建立外源性喂养的 naccarii 鲟鱼 CCs 的发育。基于这些观察结果，我们可以肯定 CCs 从孵化后的前几天就存在了。CCs 出现时，其细胞核靠近上皮的基膜，并被支持细胞包围。此外，从孵化后第 2 天开始，我们观察到具有与 CCs 高度相似特征的细胞（无结节、顶细胞质中富含线粒体和丝状物质、大量微管以及被支持细胞包裹），但其非内陷的顶表面仍保留纤毛。我们得出的结论是，这些细胞可能对应于未成熟的 CCs，其隐窝（形态分化的最终特征）尚未形成。
