Olmarker K, Danielsen N, Nordborg C, Rydevik B
Department of Anatomy, Sahlgren Hospital, Göteburg University, Sweden.
Spine (Phila Pa 1976). 1991 Jan;16(1):43-5. doi: 10.1097/00007632-199101000-00008.
The enzyme chondroitinase ABC has recently been suggested for use in chemonucleolysis. The effects of 200 U ml of chondroitinase ABC were studied on intrathecal and peripheral nerve tissue in rabbits. After the intrathecal (subarachnoid) application of 0.2 ml of either the diluent in the control group (N = 2) or chondroitinase in the study group (N = 4), no neurologic deficit was detected. Compared with the control group, no morphologic changes at the light microscopic level were induced in the spinal cord by chondroitinase. No neurophysiologic differences were detected between tibial nerves after exposure to 1 ml of the diluent (control, N = 8) or chondroitinase (the other leg) for 4 weeks, nor did the study group, compared with the control group, show any morphologic changes in the tibial nerves. Because the concentration of chondroitinase ABC tested was approximately 40 times higher than might be used clinically for chemonucleolysis, the present study indicates a wide margin of safety for unwanted side effects on nerve tissue.
最近有人提出将软骨素酶ABC用于化学髓核溶解术。研究了200 U/ml软骨素酶ABC对兔鞘内和周围神经组织的影响。在对照组（N = 2）鞘内（蛛网膜下腔）注射0.2 ml稀释剂或研究组（N = 4）鞘内注射软骨素酶后，未发现神经功能缺损。与对照组相比，软骨素酶未引起脊髓在光镜水平的形态学改变。在暴露于1 ml稀释剂（对照组，N = 8）或软骨素酶（另一条腿）4周后，胫神经之间未检测到神经生理学差异，研究组与对照组相比，胫神经也未出现任何形态学改变。由于所测试的软骨素酶ABC浓度比临床化学髓核溶解术可能使用的浓度高约40倍，本研究表明对神经组织产生不良副作用的安全范围很宽。
