α-黑素细胞刺激素对 CBA 小鼠乙酰氨基酚诱导肝毒性的影响。

Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, 10000 Zagreb, Croatia.

Molecules. 2009 Dec 2;14(12):5017-26. doi: 10.3390/molecules14125017.

Proteins and peptides in mammals are based exclusively on L-amino acids. Recent investigations show that D-amino acids exhibit physiological effects in vivo, despite of their very small quantities. We have investigated the hepatoprotective effects of the Land D-enantiomers of alpha-melanocortin peptide (alpha-MSH). The results showed that peptide-enantiomerism is related to the protective effects of melanocortin peptides in vivo. L-alpha-MSH exhibited potent hepatoprotective effect in the experimental model of acetaminophen induced hepatotoxicity in male CBA mice, while its D-mirror image was inefficient. Furthermore, the antibody to the L-peptide did not recognize the D-structure. The results indicate that the opposite peptide configuration may be used to modulate its function and metabolism in vivo and in vitro.

哺乳动物中的蛋白质和肽完全基于 L-氨基酸。最近的研究表明，尽管 D-氨基酸的含量非常少，但它们在体内仍具有生理作用。我们研究了 α-黑素细胞刺激素肽（α-MSH）的 L 型和 D 型对映体的肝保护作用。结果表明，肽对映异构体与黑素细胞刺激素肽在体内的保护作用有关。L-α-MSH 在雄性 CBA 小鼠的醋氨酚诱导肝毒性实验模型中表现出很强的肝保护作用，而其 D-对映异构体则无效。此外，针对 L-肽的抗体不能识别 D 结构。结果表明，相反的肽构型可能被用于在体内和体外调节其功能和代谢。