State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Nanxincun 20, 100093 Beijing, China.
Planta. 2010 Feb;231(3):767-77. doi: 10.1007/s00425-009-1087-z. Epub 2009 Dec 24.
Floral and vegetative development of plants is dependent on the combinatorial action of MADS-domain transcription factors. Members of the STMADS11 subclade, such as MPF1 of Physalis, are abundantly expressed in leaves as well as in floral organs, but their function is not yet clear. Our studies with transgenic Arabidopsis that over-express MPF1 suggest that MPF1 interacts with SOC1 to determine flowering time. However, MPF1 RNAi-mediated knockdown Physalis plants revealed a complex phenotype with changes in flowering time, plant architecture and seed size. Flowering of these plants was delayed by about 20% as compared to wild type. Expression of PFLFY is upregulated in the MPF1 RNAi lines, while PFFT and MPF3 genes are strongly repressed. MPF1 interacts with a subset of MADS-domain factors, namely with PFSOC1 in planta, and with PFSEP3 and PFFUL in yeast, supporting a regulatory role for this protein in flowering. The average size of seeds produced by the transgenic MPF1 RNAi plants is increased almost twofold. The height of these plants is also increased about twofold, but most axillary buds are stunted when compared to controls. Taken together, this suggests that members of the STMADS11 subclade act as positive regulators of flowering but have diverse functions in plant growth.
植物的花和营养生长依赖于 MADS 结构域转录因子的组合作用。Physalis 中的 MPF1 等 STMADS11 亚科成员在叶片和花器官中大量表达，但它们的功能尚不清楚。我们对过表达 MPF1 的转基因拟南芥的研究表明，MPF1 与 SOC1 相互作用以决定开花时间。然而，MPF1 RNAi 介导的 Physalis 植株敲低显示出复杂的表型，包括开花时间、植物结构和种子大小的变化。与野生型相比，这些植物的开花时间延迟了约 20%。MPF1 RNAi 系中 PFLFY 的表达上调，而 PFFT 和 MPF3 基因则被强烈抑制。MPF1 与一组 MADS 结构域因子相互作用，即在体内与 PFSOC1 相互作用，在酵母中与 PFSEP3 和 PFFUL 相互作用，支持该蛋白在开花中的调节作用。转基因 MPF1 RNAi 植株产生的种子平均大小增加了近两倍。这些植物的高度也增加了近两倍，但与对照相比，大多数腋芽发育不良。综上所述，这表明 STMADS11 亚科的成员作为开花的正调控因子发挥作用，但在植物生长中具有多样化的功能。
