Department of Psychiatry and Behavioral Neuroscience, University of Chicago, 924 East 57th Street, Room R018, MC 3077, Chicago, IL, 60637, USA.
Behav Genet. 2010 Mar;40(2):201-10. doi: 10.1007/s10519-009-9325-5. Epub 2009 Dec 23.
Some BALB/c substrains exhibit different levels of aggression. We compared aggression levels between male BALB/cJ and BALB/cByJ substrains using the resident intruder paradigm. These substrains were also assessed in other tests of emotionality and information processing including the open field, forced swim, fear conditioning, and prepulse inhibition tests. We also evaluated single nucleotide polymorphisms (SNPs) previously reported between these BALB/c substrains. Finally, we compared BALB/cJ and BALB/cByJ mice for genomic deletions or duplications, collectively termed copy number variants (CNVs), to identify candidate genes that might underlie the observed behavioral differences. BALB/cJ mice showed substantially higher aggression levels than BALB/cByJ mice; however, only minor differences in other behaviors were observed. None of the previously reported SNPs were verified. Eleven CNV regions were identified between the two BALB/c substrains. Our findings identify a robust difference in aggressive behavior between BALB/cJ and BALB/cByJ substrains, which could be the result of the identified CNVs.
一些 BALB/c 亚系表现出不同程度的攻击性。我们使用常驻入侵者范式比较了 BALB/cJ 和 BALB/cByJ 亚系雄性之间的攻击性水平。这些亚系还在其他情感和信息处理测试中进行了评估，包括开阔场、强迫游泳、恐惧条件反射和前脉冲抑制测试。我们还评估了之前报道的这些 BALB/c 亚系之间的单核苷酸多态性 (SNP)。最后，我们比较了 BALB/cJ 和 BALB/cByJ 小鼠的基因组缺失或重复，统称为拷贝数变异 (CNV)，以确定可能导致观察到的行为差异的候选基因。BALB/cJ 小鼠的攻击性水平明显高于 BALB/cByJ 小鼠；然而，其他行为仅观察到微小差异。之前报道的 SNP 均未得到验证。在这两个 BALB/c 亚系之间确定了 11 个 CNV 区域。我们的研究结果表明 BALB/cJ 和 BALB/cByJ 亚系之间存在攻击性行为的显著差异，这可能是由于鉴定出的 CNV 所致。
