Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Planta Med. 2010 May;76(8):831-6. doi: 10.1055/s-0029-1240725. Epub 2009 Dec 23.
Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.05-6.25 microg/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.
野葛（White Kwao Khuer）是一种药用植物，含有葛根素、大豆苷、染料木素、大豆苷元和染料木苷等主要异黄酮类化合物，具有抗衰老和雌激素作用。为了分析这些化合物，本研究使用抗葛根素和抗大豆苷多克隆抗体（PAbs）开发了一种用于总异黄酮的单酶联免疫吸附测定法（ELISA）。ELISA 法测定异黄酮的校准范围为 0.05-6.25μg/mL。新开发的检测系统测定的野葛样品中的总异黄酮浓度与 HPLC 分析结果具有良好的一致性。基于验证分析，该 ELISA 分析方法是一种精确、准确、灵敏的测定野葛总异黄酮的方法。
