[反相高效液相色谱法分离测定粉葛茎叶中葛根素、大豆苷和大豆苷元]
[Separation and determination of puerarin, daidzin and daidzein in stems and leaves of Pueraria thomsonii by RP-HPLC].
作者信息
Zhou Hong-Ying, Wang Jian-Hua, Yan Feng-Yun
机构信息
College of Agronomy, Shandong Agricultural University, Taian 271018, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 May;32(10):937-9.
OBJECTIVE
To separation and determine the contents of puerarin, daidzin and daidzein in the stems and the leaves of Pueraria thomsonii, and to provide scientific basis for developing and using of the stems and the leaves.
METHOD
A RP-HPLC method was applied with a Diamonsil C18 column (4.6 mm x 150 mm, 5 microm) by gradient elution using methanol-1% glacial acetic acid solution as the mobil phase. The flow rate was 1 mL x min(-1) and the detective wavelength was 250 nm, the column temperature was 25 degrees C.
RESULT
All of the three compounds showed good linearities (r >0.9995) and the recoveries were in the range of 99.0% - 101.6%. The contents of puerarin, daidzin and daidzein in the stems are higher than those in the leaves.
CONCLUSION
The method was accurate and could be used to contral the quality of the stems and leaves of P. thomsonii.
目的
分离并测定甘葛藤茎和叶中葛根素、大豆苷和大豆苷元的含量，为甘葛藤茎和叶的开发利用提供科学依据。
方法
采用RP-HPLC法，以Diamonsil C18柱（4.6 mm×150 mm，5μm）为分离柱，以甲醇-1%冰醋酸溶液为流动相进行梯度洗脱。流速为1 mL·min-1，检测波长为250 nm，柱温为25℃。
结果
三种化合物线性关系良好（r＞0.9995），回收率在99.0%～101.6%之间。甘葛藤茎中葛根素、大豆苷和大豆苷元的含量高于叶中。
结论
该方法准确可靠，可用于控制甘葛藤茎和叶的质量。
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