半夏与水半夏的RAPD分析及特异性DNA探针的构建
[RAPD analysis and construction of specific DNA probes between Pinellia ternata and Typhonium flagelliforme].
作者信息
Chen Guo, Bai Quan, Wang Chao-li, Ren Bi-xuan, Tang En-jie, Feng Li, Jing Bao-qian, Hu Wei-min
机构信息
Institute of Immunology & Molecular Biology, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
出版信息
Zhong Yao Cai. 2009 Sep;32(9):1374-5.
OBJECTIVE
To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme.
METHODS
Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes and then conducted Southern blot.
RESULTS
A great deal of special bands was found between Pinellia ternata and Typhonium flagelliforme. A Pinellia ternata specific molecule was screened.
CONCLUSION
RAPD analysis and specific DNA probes show potential value in the identification of Pinellia ternata and Typhonium flagelliforme.
目的
建立一种鉴别半夏和水半夏的分子标记方法。
方法
采用22个随机寡核苷酸引物对四川两种半夏和广西两种水半夏的基因组DNA进行RAPD分析。对特异片段进行测序，标记为探针，然后进行Southern杂交。
结果
半夏和水半夏之间发现大量特异条带。筛选出一种半夏特异分子。
结论
RAPD分析和特异DNA探针在半夏和水半夏的鉴别中显示出潜在价值。
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