[南板蓝根种质资源的随机扩增多态性DNA分析]
[RAPD analysis on the germplasm resources of Baphicacanthus cusia].
作者信息
Huang Yu-ji, Chen Jing-ying
机构信息
Institute of Agrobiological resources, Fujian Academy of Agricultural Sciences/Medicinal Plant Research Center, Fuzhou 350003, China.
出版信息
Zhong Yao Cai. 2010 Feb;33(2):183-6.
OBJECTIVE
To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve.
METHODS
The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.
RESULTS
104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions.
CONCLUSION
There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.
目的
在分子水平上探索马蓝种质资源的遗传多样性。
方法
采用分子生物学技术——随机扩增多态性DNA（RAPD）。
结果
筛选104条随机十聚体引物用于马蓝的RAPD片段分析。12条引物扩增出422条DNA带，其中55.69%的产物具有多态性。基于UPGMA聚类分析，构建DNA分子树状图以探讨马蓝种质资源的遗传多样性。遗传差异在一定程度上与形态差异相关，但与地理区域无关。
结论
不同自然居群的马蓝在分子水平上确实存在丰富的遗传多样性。
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