Gulani Jatinder, Norbury Christopher C, Bonneau Robert H, Beckwith Catherine S
Department of Comparative Medicine, Penn State Hershey College of Medicine, Hershey, Pennsylvania, USA.
Comp Med. 2009 Dec;59(6):534-44.
Infection of mice with Helicobacter hepaticus is common in research colonies, yet little is known about how this persistent infection affects immunologic research. The goal of this study was to determine whether H. hepaticus infection status can modulate immune responses specific to herpes simplex virus type 1 (HSV1) and the phenotypic and functional characteristics of dendritic cells (DC) of mice. We compared virus-specific antibody and T cell-mediated responses in H. hepaticus-infected and noninfected mice that were inoculated intranasally with HSV1. The effect of H. hepaticus on the HSV1-specific antibody and T cell-mediated immune responses in superficial cervical and tracheobronchal lymph nodes (LN) did not reach statistical significance. Surface expression of the maturation-associated markers CD40, CD80, CD86, and MHC II and percentages of IL12p40- and TNFalpha-producing DC from spleen and colic LN in H. hepaticus-infected mice and noninfected mice were measured in separate experiments. Expression of CD40, CD86, and MHC II and percentages of IL12p40- and TNFalpha-producing DC from colic LN were decreased in H. hepaticus-infected mice. In contrast, H. hepaticus infection did not reduce the expression of these molecules by splenic DC. Expression of CD40, CD80, CD86, and MHC II on splenic DC from H. hepaticus-infected mice was increased after in vitro lipopolysaccharide stimulation. These results indicate that H. hepaticus infection can influence the results of immunologic assays in mice and support the use of H. hepaticus-free mice in immunologic research.
在研究群体中，小鼠感染肝螺杆菌很常见，但对于这种持续性感染如何影响免疫学研究却知之甚少。本研究的目的是确定肝螺杆菌感染状态是否能调节小鼠针对1型单纯疱疹病毒（HSV1）的免疫反应以及树突状细胞（DC）的表型和功能特征。我们比较了经鼻接种HSV1的肝螺杆菌感染小鼠和未感染小鼠的病毒特异性抗体和T细胞介导的反应。肝螺杆菌对浅表颈淋巴结和气管支气管淋巴结（LN）中HSV1特异性抗体和T细胞介导的免疫反应的影响未达到统计学显著性。在单独的实验中，测量了肝螺杆菌感染小鼠和未感染小鼠脾脏和结肠淋巴结中成熟相关标志物CD40、CD80、CD86和MHC II的表面表达以及产生IL12p40和TNFα的DC的百分比。肝螺杆菌感染小鼠结肠淋巴结中CD40、CD86和MHC II的表达以及产生IL12p40和TNFα的DC的百分比降低。相比之下，肝螺杆菌感染并未降低脾脏DC中这些分子的表达。体外脂多糖刺激后，肝螺杆菌感染小鼠脾脏DC上CD40、CD80、CD86和MHC II的表达增加。这些结果表明，肝螺杆菌感染可影响小鼠免疫学检测结果，并支持在免疫学研究中使用无肝螺杆菌的小鼠。
