Hong Mee Sook, Park Hun-Kuk, Yang Jong-Soo, Park Hi-Joon, Kim Seung-Tae, Kim Seung-Nam, Park Ji-Yeun, Song Jeong Yoon, Park Hyun-Kyung, Jo Dae Jean, Park Sung Wook, HwanYun Dong, Ban Ju Yeon, Chung Joo-Ho
Kohwang Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea.
Neurol Res. 2010 Feb;32 Suppl 1:74-8. doi: 10.1179/016164109X12537002794165.
To find new biomarkers by stimulating acupuncture point GB34 (Yangneungcheon) which has neuroprotective effect on the mouse model of Parkinson's disease, analysis of cDNA microarray on mRNAs of the substantia nigra was performed.
Male C57BL/6 mice were divided into two groups: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice (MPTP group, n=3); 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and acupuncture (GB34)-treated mice (MPTP + ACU group, n=3). The mice received an intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (30 mg/kg) once daily for 3 consecutive days. Manual acupuncture was performed 2 hours after every injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The total RNA in the substantia nigra of each mouse was isolated on 3 days after the last 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection. Agilent mouse whole genome 44K chip was used for microarray analysis and the hybridization image was analysed by GenePix Pro 6.0. Data normalization and analysis were performed using GeneSpring GX 7.3.1 program.
The acupuncture stimulation revealed 799 genes (424 up- and 375 down-regulated) of which expression levels were changed more than two-folds in the MPTP + ACU group, compared to the MPTP group. The genes selected were classified into several categories based on their functions using DAVID Bioinformatics Resources 2008 (http://david.abcc.ncifcrf.gov/) and KEGG PATHWAY Database (http://www.genome.jp/kegg/pathway.html).
Biomarkers in response to acupuncture stimulation to GB34 were identified in a mouse model for Parkinson's disease. These biomarkers might provide a promising clue for understanding the neuroprotective effect of acupuncture in Parkinson's disease.
通过刺激对帕金森病小鼠模型具有神经保护作用的穴位GB34（阳陵泉）来寻找新的生物标志物，对黑质mRNA进行cDNA微阵列分析。
将雄性C57BL/6小鼠分为两组：1-甲基-4-苯基-1,2,3,6-四氢吡啶处理的小鼠（MPTP组，n = 3）；1-甲基-4-苯基-1,2,3,6-四氢吡啶和针刺（GB34）处理的小鼠（MPTP + ACU组，n = 3）。小鼠连续3天每天腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶（30 mg/kg）。每次注射1-甲基-4-苯基-1,2,3,6-四氢吡啶后2小时进行手动针刺。在最后一次注射1-甲基-4-苯基-1,2,3,6-四氢吡啶3天后，分离每只小鼠黑质中的总RNA。使用安捷伦小鼠全基因组44K芯片进行微阵列分析，并通过GenePix Pro 6.0分析杂交图像。使用GeneSpring GX 7.3.1程序进行数据标准化和分析。
针刺刺激显示，与MPTP组相比，MPTP + ACU组中有799个基因（424个上调和375个下调）的表达水平变化超过两倍。使用DAVID生物信息学资源2008（http://david.abcc.ncifcrf.gov/）和KEGG通路数据库（http://www.genome.jp/kegg/pathway.html）根据其功能将所选基因分类为几类。
在帕金森病小鼠模型中鉴定了对GB34针刺刺激有反应的生物标志物。这些生物标志物可能为理解针刺在帕金森病中的神经保护作用提供有希望的线索。
