Yeo Sujung, An Keon Sang, Hong Yeon-Mi, Choi Yeong-Gon, Rosen Bruce, Kim Sung-Hoon, Lim Sabina
Research Group of Pain and Neuroscience, WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyung Hee University, Seoul, Republic of Korea . ; Department of Basic Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea .
Department of Basic Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea .
Genet Mol Biol. 2015 Mar;38(1):115-27. doi: 10.1590/S1415-475738120140137. Epub 2014 Mar 17.
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the death of dopamine-generating cells in the substantia nigra (SN). Acupuncture stimulation results in an enhanced survival of dopaminergic neurons in the SN in Parkinsonism animal models. The present study investigated changes in gene expression profiles measured using whole transcript array in the SN region related to the inhibitory effects of acupuncture in a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Parkinsonism model. In this model, acupuncture stimulation at GB34 and LR3 attenuated the decrease in tyrosine hydroxylase in the SN region; stimulation at non-acupoints did not suppress this decrease. Gene array analysis revealed that 22 (10 annotated genes: Cdh1, Itih2, Mpzl2, Rdh9, Serping1, Slc6a13, Slc6a20a, Slc6a4, Tph2, and Ucma) probes that were up-regulated in MPTP animals relative to controls were exclusively down-regulated by acupuncture stimulation. In addition, 17 (two annotated genes: 4921530L21Rik and Gm13931) probes that were down-regulated in MPTP animals compared to controls were exclusively up-regulated by acupuncture stimulation. These findings indicate that the 39 probes (12 annotated genes) affected by MPTP and acupuncture may be responsible for the inhibitory effects of acupuncture on degeneration-related gene expression in the SN following damage induced by MPTP intoxication.
帕金森病（PD）是一种神经退行性疾病，其特征是黑质（SN）中产生多巴胺的细胞死亡。在帕金森病动物模型中，针刺刺激可提高黑质中多巴胺能神经元的存活率。本研究在慢性1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）帕金森病模型中，研究了使用全转录组阵列测量的SN区域基因表达谱的变化，这些变化与针刺的抑制作用有关。在该模型中，针刺刺激足少阳胆经34穴（GB34）和足厥阴肝经3穴（LR3）可减轻SN区域酪氨酸羟化酶的减少；非穴位刺激则不能抑制这种减少。基因阵列分析显示，与对照组相比，MPTP处理动物中上调的22个（10个注释基因：Cdh1、Itih2、Mpzl2、Rdh9、Serping1、Slc6a13、Slc6a20a、Slc6a4、Tph2和Ucma）探针仅在针刺刺激后下调。此外，与对照组相比，MPTP处理动物中下调的17个（2个注释基因：4921530L21Rik和Gm13931）探针仅在针刺刺激后上调。这些发现表明，受MPTP和针刺影响的39个探针（12个注释基因）可能是针刺对MPTP中毒诱导损伤后SN中与变性相关基因表达的抑制作用的原因。
