Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest 050568, Romania.
Biochem Biophys Res Commun. 2010 Jan 15;391(3):1443-8. doi: 10.1016/j.bbrc.2009.12.089. Epub 2009 Dec 23.
Resistin and high glucose (HG) are concomitantly present at elevated concentration in diabetic's plasma; both are pro-inflammatory agents acting on vascular cells by mechanisms that are not fully understood. We questioned whether resistin and HG affect the expression of major adhesion molecules, P-selectin and fractalkine in human endothelial cells (HEC). The results showed that in HEC (i) resistin increased P-selectin expression; (ii) HG up-regulated Fk expression; (iii) P-selectin and fractalkine were functional increasing monocyte adhesion to activated cells. Co-stimulation with resistin and HG increased P-selectin and fractalkine mRNA and protein and induced monocyte adhesion, generated an increase in NADPH oxidase activity and of the intracellular reactive oxygen species and activated the NF-kB and AP-1 transcription factors at similar values as those of each activator. In conclusion in HEC, resistin and HG induce the up-regulation of P-selectin and fractalkine and the ensuing increased monocyte adhesion by a mechanism involving oxidative stress and NF-kB and AP-1 activation.
抵抗素和高葡萄糖（HG）在糖尿病患者的血浆中同时存在于升高的浓度；两者都是通过尚未完全了解的机制作用于血管细胞的促炎剂。我们质疑抵抗素和 HG 是否会影响人内皮细胞（HEC）中主要粘附分子 P-选择素和 fractalkine 的表达。结果表明，在 HEC 中：（i）抵抗素增加 P-选择素的表达；（ii）HG 上调 Fk 的表达；（iii）P-选择素和 fractalkine 使单核细胞对激活细胞的粘附功能增强。抵抗素和 HG 的共刺激增加了 P-选择素和 fractalkine mRNA 和蛋白的表达，并诱导单核细胞的黏附，产生 NADPH 氧化酶活性和细胞内活性氧的增加，并激活 NF-kB 和 AP-1 转录因子，其活性与每个激活剂的值相似。总之，在 HEC 中，抵抗素和 HG 通过涉及氧化应激和 NF-kB 和 AP-1 激活的机制诱导 P-选择素和 fractalkine 的上调以及随后单核细胞的粘附增加。
