Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, Brazil.
Toxicon. 2010 Jun 1;55(6):1100-6. doi: 10.1016/j.toxicon.2009.12.011. Epub 2009 Dec 23.
In the present study, it was investigated which components are responsible for the anti-inflammatory properties of Crotalus durissus terrificus venom (CdtV). The effect of crotoxin, as well as of other CdtV components was evaluated on edema, cell migration and alterations in leukocyte-endothelium interactions induced by carrageenan. Crotoxin (40 microg kg(-1)) was injected at different time periods before or after the injection of carrageenan (15 mg kg(-1)) into the mouse hind paw, peritoneum or scrotum. Results showed that crotoxin, but not other CdtV components, significantly inhibited inflammatory edema and cell migration when administered before or after carrageenan injection in mice. This toxin also prevented the occurrence of alterations in leukocyte-endothelium interactions induced by carrageenan injection, such as the increase in adhered cells. In animals pretreated with Boc2 (a selective antagonist of formyl peptide receptors), crotoxin showed neither inhibitory effects on edema and cell migration, nor prevented alterations in leukocyte-endothelium interactions induced by carrageenan. These findings demonstrate that crotoxin is the component responsible for the long-lasting anti-inflammatory activity of crude C. durissus terrificus venom, and activation of formyl peptide receptors seems to play a major role in this effect.
在本研究中，研究了响尾蛇蛇毒（CdtV）的抗炎特性的哪些成分负责。评价了 crotoxin 以及其他 CdtV 成分对卡拉胶诱导的水肿、细胞迁移和白细胞-内皮细胞相互作用改变的影响。在向小鼠后爪、腹膜或阴囊注射卡拉胶（15mg/kg）之前或之后的不同时间点，注射 crotoxin（40μg/kg）。结果表明，crotoxin 但不是其他 CdtV 成分，在向小鼠注射卡拉胶之前或之后给药时，可显著抑制炎症性水肿和细胞迁移。该毒素还可防止卡拉胶注射引起的白细胞-内皮细胞相互作用的改变，如粘附细胞的增加。在预先用 Boc2（一种选择性的甲酰肽受体拮抗剂）处理的动物中，crotoxin 既没有抑制水肿和细胞迁移的作用，也没有防止卡拉胶诱导的白细胞-内皮细胞相互作用的改变。这些发现表明 crotoxin 是导致粗 C. durissus terrificus 毒液具有持久抗炎活性的成分，而甲酰肽受体的激活似乎在这种作用中起主要作用。
