Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China.
Virus Res. 2010 Mar;148(1-2):60-70. doi: 10.1016/j.virusres.2009.12.004. Epub 2009 Dec 23.
Classical swine fever virus (CSFV) is an etiologic agent that causes a highly contagious disease in pigs. Laying a foundation to solve problems in its pathogenic mechanism, microarray analysis was performed to detect the gene transcriptional profiles in peripheral blood mononuclear cells (PBMC) following infection with a Chinese highly virulent CSFV strain Shimen. Three susceptible pigs were inoculated intramuscularly with a lethal dose (1.0x10(6) TCID(50)) of CSFV. Pigs showed classical CSF signs, depletion of lymphocytes and monocytes consistent with CSFV infection, and the CSFV genome was also confirmed in the PBMC. The PBMC were isolated at 1, 3, 6 and 9 days post-inoculation (dpi). Total RNA were extracted and subjected to microarray analysis. Data showed that expression of 847 genes wherein 467 genes were known function and the remaining 380 genes were unknown function, and 541 up- and 306 down-regulation, altered after infection. There were 54, 181, 438 and 354 up- and 61, 120, 218 and 145 down-regulated genes presented on 1, 3, 6 and 9dpi, respectively. These genes were involved in immune response (14.5%), apoptosis (3.3%), signal transduction (7.6%), transcription (4.4%), metabolism (11%), transport (3.9%), development (6.8%) and cell cycle (3.7%). Results demonstrated its usefulness in exploring the pathogenic mechanisms of CSFV.
经典猪瘟病毒（CSFV）是一种引起猪高度传染性疾病的病原体。为了解决其致病机制中的问题，我们进行了微阵列分析，以检测感染中国高毒力 CSFV 毒株石门后外周血单核细胞（PBMC）中的基因转录谱。将 3 头易感猪肌肉内接种致死剂量（1.0x10(6)TCID(50))的 CSFV。猪表现出典型的 CSF 症状，淋巴细胞和单核细胞耗竭，符合 CSFV 感染，PBMC 中也证实存在 CSFV 基因组。感染后 1、3、6 和 9 天分离 PBMC。提取总 RNA 并进行微阵列分析。数据显示，847 个基因的表达发生了变化，其中 467 个基因具有已知功能，其余 380 个基因具有未知功能，541 个上调和 306 个下调。感染后分别有 54、181、438 和 354 个上调和 61、120、218 和 145 个下调基因在 1、3、6 和 9dpi 时出现。这些基因参与免疫反应（14.5%）、凋亡（3.3%）、信号转导（7.6%）、转录（4.4%）、代谢（11%）、运输（3.9%）、发育（6.8%）和细胞周期（3.7%）。结果表明，它在探索 CSFV 的致病机制方面具有实用性。
