College of Science, Northeastern University, Shenyang, People's Republic of China.
J Gen Virol. 2010 Sep;91(Pt 9):2254-62. doi: 10.1099/vir.0.022020-0. Epub 2010 May 12.
Leukopenia and immunosuppression are characteristic clinical manifestations of classical swine fever and peripheral blood mononuclear cells (PBMCs) are major targets of classical swine fever virus. To investigate proteomic expression changes in swine PBMCs during lethal CSFV infection, proteins of PBMCs from five lethally CSFV-infected pigs were resolved by two-dimensional electrophoresis followed by mass spectrometry. Quantitative intensity analysis revealed that 66 protein spots showed altered expression, 44 of which were identified as 34 unique proteins by MALDI-TOF-MS/MS. Cellular functions of these proteins included cytoskeletal, energy metabolism, protein translation and processing, antioxidative stress, heat shock and blood clotting. Western blot analysis confirmed the upregulation of annexin A1 and downregulation of cofilin. Identification of these changed levels of expression provides an understanding at the molecular level of the response of in vivo target cells to CSFV infection and of the pathogenic mechanisms of leukopenia and immunosuppression induced by the virus.
白细胞减少症和免疫抑制是经典猪瘟的特征性临床表现，外周血单个核细胞（PBMCs）是经典猪瘟病毒的主要靶细胞。为了研究致死性 CSFV 感染期间猪 PBMCs 的蛋白质组表达变化，通过二维电泳和质谱法对来自 5 头致死性 CSFV 感染猪的 PBMCs 蛋白质进行了分离。定量强度分析显示，66 个蛋白斑点的表达发生改变，其中 44 个通过 MALDI-TOF-MS/MS 鉴定为 34 个独特蛋白。这些蛋白质的细胞功能包括细胞骨架、能量代谢、蛋白质翻译和加工、抗氧化应激、热休克和血液凝固。Western blot 分析证实了 annexin A1 的上调和 cofilin 的下调。这些表达变化水平的鉴定提供了对体内靶细胞对 CSFV 感染的反应以及病毒引起的白细胞减少症和免疫抑制的发病机制的分子水平的理解。
