Zangerie P F, Calberg-Bacq C M, Colin C, Franchimont P, François C, Gosselin L, Kozma S, Osterrieth P M
Cancer Res. 1977 Dec;37(12):4326-31.
Murine mammary tumor virus main glycoprotein (gp47), prepared by diethylaminoethyl cellulose and hydroxyapatite chromatography of detergent-mercaptoethanol-KCI-disrupted virion, was used as labeled antigen in a highly specific and reproducible radioimmunoassay. Seven other (glyco) proteins of the virus were antigenically distinct from gp47. Serum and organs of unifected C57BL mice did not contain gp47, but sera of infected Swiss and RIII mice did contain the antigen. Despite the high content in the mammary gland, the level of gp47 in other organs was identical in male and female mice. The titer of gp47 in serum was high in tumor-bearing females, but it varied with the mouse strain. Anti-gp47 immunoglobulins could not be detected. The investigation included 314 human sera (107 normal, 65 benign mastopathy, 89 breast vancer, and 53 digestive cancer). None contained an antigen related to gp47. One of 20 human mammary cyst fluids was positive.
通过用二乙氨基乙基纤维素和羟基磷灰石对经去污剂-巯基乙醇-KCl处理而裂解的病毒粒子进行层析制备的鼠乳腺肿瘤病毒主要糖蛋白（gp47），被用作一种高度特异性且可重复的放射免疫分析中的标记抗原。该病毒的其他七种（糖基）蛋白在抗原性上与gp47不同。未感染的C57BL小鼠的血清和器官不含gp47，但感染的瑞士小鼠和RIII小鼠的血清确实含有该抗原。尽管乳腺中含量很高，但gp47在雄性和雌性小鼠其他器官中的水平是相同的。荷瘤雌性小鼠血清中gp47的滴度很高，但因小鼠品系而异。未检测到抗gp47免疫球蛋白。该研究包括314份人血清（107份正常血清、65份良性乳腺病血清、89份乳腺癌血清和53份消化道癌血清）。均未含有与gp47相关的抗原。20份人乳腺囊肿液中有1份呈阳性。
