Osterrieth P M, Kozma S, Hendrick J C, François C, Calberg-Bacq C M, Franchimont P, Gosselin L
J Gen Virol. 1979 Oct;45(1):41-50. doi: 10.1099/0022-1317-45-1-41.
Extracts of various organs, mammary tumours and sera from milk-borne MMTV infected Swiss albino mice of different age, sex and physiological conditions were tested by radioimmunoassay for the presence of gp47, the main envelope polypeptide, and p28, the main core protein of the virus. Except in brain, ovaries and testes, both antigens were found in all organs of old animals and of females after the onset of their first pregnancy. Antigens were not present in organs of weanlings or in whole foetuses. Higher values were found in mammary glands, mammary tumours, epididymis and seminal vesicles. These organs also harboured a greater amount of gp47 than p28. The serum generally contained gp47 but rarely p28. This indicates that gp47 is not virion-bound in blood. Pregnancy, lactation and especially the presence of mammary tumours increased the concentration of gp47 in serum. The results do not allow localization of target organs of MMTV infection in the interval between ingestion of the virus by the suckling mouse and the first pregnancy. Moreover, results obtained with one group of mice devoid of exogenous virus show that, as endogenous MMTV genome expresses p28, it might account for part of the p28 detected exogenous MMTV-infected mice.
通过放射免疫分析法，对来自不同年龄、性别和生理状况的经乳汁传播的小鼠乳腺肿瘤病毒（MMTV）感染的瑞士白化小鼠的各种器官、乳腺肿瘤和血清进行检测，以确定主要包膜多肽gp47和病毒主要核心蛋白p28的存在情况。除了脑、卵巢和睾丸外，在老年动物以及首次怀孕后的雌性动物的所有器官中均发现了这两种抗原。在断奶小鼠的器官或整个胎儿中未发现抗原。在乳腺、乳腺肿瘤、附睾和精囊中发现的值较高。这些器官中gp47的含量也比p28多。血清中一般含有gp47，但很少含有p28。这表明gp47在血液中不与病毒粒子结合。怀孕、哺乳，尤其是乳腺肿瘤的存在会增加血清中gp47的浓度。这些结果无法确定在幼鼠摄入病毒到首次怀孕这段时间内MMTV感染的靶器官。此外，对一组不含外源病毒的小鼠的检测结果表明，由于内源性MMTV基因组表达p28，它可能是外源MMTV感染小鼠中检测到的部分p28的来源。
