[A rapid colorimetric assay for viability of osteosarcoma cells after chemotherapy].
作者信息
Chen Qiang, Zhang Jin-hua, Chen Jian-ting, Shi Zhan-jun, Deng Yong-jian
机构信息
Department of Spinal and Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Dec;29(12):2490-1.
OBJECTIVE
To introduce a rapid colorimetric method for assessing the viability of osteosarcoma cells after chemotherapy.
METHODS
Colorimetric assay and automatic microplate scanning spectrophotometer were used for assaying the viability of osteosarcoma cells.
RESULTS
Close correlation was found between the absorbance at 570 nm of the formazan products and the number of viable osteosarcoma cells.
CONCLUSION
An effective, sensitive and convenient colorimetric assay has been established to assess the survival of osteosarcoma cells following chemotherapy.
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