Mi Yuling, Zhang Caiqiao, Li Chun Mei, Taneda Shinji, Watanabe Gen, Suzuki Akira K, Taya Kazuyoshi
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology.
J Reprod Dev. 2010 Apr;56(2):195-9. doi: 10.1262/jrd.09-074n. Epub 2009 Dec 25.
The 4-nitrophenol (PNP) in diesel exhaust particles (DEP) has been identified as a vasodilator and is a known degradation product of the insecticide parathion. In this study, the protective effect of quercetin, a potent oxygen free radical scavenger and metal chelator, against the oxidative damage of PNP on cultured testicular cells was studied in male embryonic chickens. Testicular cells from Day 18 embryos were cultured in serum-free McCoy's 5A medium and challenged with quercetin (1.0 microg/ml) alone or in combinations with PNP (10(-7)-10(-5) M) for 48 h. The oxidative damage was estimated by measuring cell viability, content of malondialdehyde (MDA), activity of superoxide dismutase (SOD) and glutathione peroxidation (GSH-Px) activity. The results showed that exposure to PNP (10(-5) M) induced condensed nuclei, vacuolated cytoplasm and a decrease in testicular cell viability and spermatogonial cell number. Exposure to PNP induced lipid peroxidation by elevation of the content of MDA. Exposure to PNP also decreased GSH-Px activity and SOD activity. However, simultaneous supplementation with quercetin restored these parameters to the same levels as the control. Consequently, PNP induced oxidative stress in spermatogonial cells, and dietary quercetin may attenuate the reproductive toxicity of PNP to restore the intracellular antioxidant system in the testicular cells of embryonic chickens.
柴油废气颗粒（DEP）中的4-硝基苯酚（PNP）已被确认为一种血管舒张剂，并且是杀虫剂对硫磷的已知降解产物。在本研究中，在雄性胚胎鸡中研究了槲皮素（一种有效的氧自由基清除剂和金属螯合剂）对PNP对培养的睾丸细胞氧化损伤的保护作用。将来自18日龄胚胎的睾丸细胞在无血清的 McCoy's 5A培养基中培养，并单独用槲皮素（1.0微克/毫升）或与PNP（10^(-7)-10^(-5) M）联合处理48小时。通过测量细胞活力、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GSH-Px）活性来评估氧化损伤。结果表明，暴露于PNP（10^(-5) M）会诱导细胞核浓缩、细胞质空泡化，并导致睾丸细胞活力和精原细胞数量减少。暴露于PNP会通过提高MDA含量诱导脂质过氧化。暴露于PNP还会降低GSH-Px活性和SOD活性。然而，同时补充槲皮素可将这些参数恢复到与对照相同的水平。因此，PNP在精原细胞中诱导氧化应激，而膳食中的槲皮素可能会减轻PNP的生殖毒性，以恢复胚胎鸡睾丸细胞中的细胞内抗氧化系统。
