Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Anat Rec (Hoboken). 2013 Oct;296(10):1650-7. doi: 10.1002/ar.22765. Epub 2013 Aug 30.
The beneficial effects of quercetin on reproductive damage elicited by 4-nitrophenol (PNP) were studied in adult male mice. A six-week treatment of weekly intraperitoneal injections of PNP (50 mg/kg) resulted in severe damage to the seminiferous tubules, a remarkable increase in both hydroxyl radical and malondiadehyde production, and notably decreased glutathione peroxidase and superoxide dismutase activities. Moreover, PNP treatment induced germ cell apoptosis, inhibited Bcl-xl expression, and then activated Bax expression and the caspase-3 enzyme. Exposure to PNP also increased XBP-1 and HO-1 mRNAs levels. However, simultaneous supplementation with quercetin (75 mg/kg) attenuated the toxicity induced by PNP through renewal of the antioxidant enzyme's status, alleviating apoptosis by regulating the expressions of Bax and Bcl-xl, XBP-1 and HO-1mRNAs, and the regulation of caspase-3 activity. Taken together, these findings indicated that the antioxidant quercetin displays a potential preventive effect on PNP-induced oxidative damage in mouse testes and may represent an efficient supplement to attenuate reproductive toxicity from environmental toxicants in order to ensure reproductive health and sperm production.
研究了槲皮素对 4-硝基苯酚（PNP）引起的雄性成年小鼠生殖损伤的有益作用。每周腹腔注射 PNP（50mg/kg）6 周的治疗导致生精小管严重损伤，羟自由基和丙二醛生成显著增加，谷胱甘肽过氧化物酶和超氧化物歧化酶活性明显降低。此外，PNP 处理诱导生殖细胞凋亡，抑制 Bcl-xl 表达，然后激活 Bax 表达和 caspase-3 酶。PNP 处理还增加了 XBP-1 和 HO-1 mRNA 水平。然而，同时补充槲皮素（75mg/kg）通过恢复抗氧化酶的状态减轻了 PNP 引起的毒性，通过调节 Bax 和 Bcl-xl、XBP-1 和 HO-1mRNA 的表达以及 caspase-3 活性的调节来缓解凋亡。总之，这些发现表明抗氧化剂槲皮素对 PNP 诱导的小鼠睾丸氧化损伤具有潜在的预防作用，可能是一种有效的补充剂，可以减轻环境毒物的生殖毒性，以确保生殖健康和精子生成。
