Takei Shiro, Tokuhira Yu, Shimada Atsuyoshi, Hosokawa Masanori, Fukuoka Shin-Ichi
Department of Pathology, Institute of Developmental Research, Aichi Human Service Center, Kasugai, Japan.
Anat Sci Int. 2010 Dec;85(4):245-50. doi: 10.1007/s12565-009-0067-5. Epub 2009 Dec 25.
We developed a novel method for enhancing light-microscopic visualization of pancreatic zymogen granules in a selective manner on hematoxylin and eosin-stained sections. By using an absorption filter that transmits light with wavelength from 510 to 550 nm, corresponding to the narrow absorption spectrum of eosin, only eosinophilic tissue and cellular components were remarkably highlighted as distinct shadows against lighter background consisting of basophilic components. Using a pair of mirror sections of the pancreas, immunocytochemistry with anti-amylase antibody confirmed that the shadows observed through the filter represented zymogen granules. Immersion in formalin for 36 h at room temperature was the optimal fixation condition. Here we designate the procedure as the "eosin-shadow method" and propose that this technique is convenient and useful to help investigators identify zymogen granules more easily in routine pathological examination and histological studies.
我们开发了一种新方法，可在苏木精和伊红染色切片上以选择性方式增强胰腺酶原颗粒的光学显微镜可视化。通过使用一个吸收滤光片，该滤光片可透射波长为510至550 nm的光，这与伊红的窄吸收光谱相对应，只有嗜酸性组织和细胞成分作为明显的阴影在由嗜碱性成分组成的较浅背景下显著突出。使用一对胰腺的镜像切片，用抗淀粉酶抗体进行免疫细胞化学证实，通过滤光片观察到的阴影代表酶原颗粒。在室温下于福尔马林中浸泡36小时是最佳固定条件。在此我们将该程序命名为“伊红阴影法”，并提出该技术方便且有用，有助于研究人员在常规病理检查和组织学研究中更轻松地识别酶原颗粒。
