Pasyk K A, Hassett C A
Department of Surgery, University of Michigan Medical School, Ann Arbor.
Pathol Res Pract. 1989 Jun;184(6):635-8. doi: 10.1016/S0344-0338(89)80170-0.
A modified hematoxylin and eosin staining method for glutaraldehyde fixed, osmium tetroxide postfixed, and epoxy resin (Medcast Resin) embedded tissue is described. Two microns thick sections of human and animal tissues are treated with 4% H2O2 for 4 minutes, washed, dryed, and then flooded with Gill's III hematoxylin in a moist chamber at 37 degrees C for 90 minutes. Subsequent steps are: washing; bluing in ammonia water for 1 minutes; washing again and staining in 1% eosin Y alcoholic solution for 5 minutes; rinsing in 100% ethanol; drying and mounting with Permont. This procedure is quick, easy and successful in demonstrating both color scale and quality similar to hematoxylin and eosin stains obtained in standard paraffin sections.
描述了一种用于戊二醛固定、四氧化锇后固定及环氧树脂（Medcast树脂）包埋组织的改良苏木精和伊红染色方法。将人和动物组织的2微米厚切片用4%过氧化氢处理4分钟，冲洗、干燥，然后在37℃的湿盒中用吉尔氏III苏木精覆盖90分钟。后续步骤为：洗涤；在氨水中变蓝1分钟；再次洗涤并在1%伊红Y酒精溶液中染色5分钟；在100%乙醇中冲洗；干燥并用Permont封固。该方法快速、简便，能成功显示出与标准石蜡切片中苏木精和伊红染色相似的色阶和质量。
