Modified hematoxylin and eosin staining method for epoxy-embedded tissue sections.

A modified hematoxylin and eosin staining method for glutaraldehyde fixed, osmium tetroxide postfixed, and epoxy resin (Medcast Resin) embedded tissue is described. Two microns thick sections of human and animal tissues are treated with 4% H2O2 for 4 minutes, washed, dryed, and then flooded with Gill's III hematoxylin in a moist chamber at 37 degrees C for 90 minutes. Subsequent steps are: washing; bluing in ammonia water for 1 minutes; washing again and staining in 1% eosin Y alcoholic solution for 5 minutes; rinsing in 100% ethanol; drying and mounting with Permont. This procedure is quick, easy and successful in demonstrating both color scale and quality similar to hematoxylin and eosin stains obtained in standard paraffin sections.