Department of Primary Industries and Fisheries, Bribie Island Aquaculture Research Centre, QLD, 4507, Australia.
Fish Physiol Biochem. 2005 Apr;31(2-3):149-52. doi: 10.1007/s10695-006-0017-2.
This paper reports the isolation of two putative D(2)R promoters from grey mullet, one 5'flanking and the other an intronic sequence immediately upstream of the first coding exon. Promoter activity of the intronic sequence was confirmed in vitro through functional analysis using luciferase as reporter gene. The functional characteristics of the region flanking the 5'UTR is currently under investigation.
本文报道了从灰鲻中分离出两个假定的 D(2)R 启动子,一个位于 5'侧翼,另一个位于第一个编码外显子的上游内含子序列。通过使用荧光素酶作为报告基因的体外功能分析,证实了内含子序列的启动子活性。目前正在研究侧翼 5'UTR 区域的功能特征。