Şimşek Emrah
Erciyes Üniversitesi Veteriner Fakültesi, Su Ürünleri ve Hastalıkları Anabilim Dalı, Kayseri, Türkiye
Turkiye Parazitol Derg. 2019 Sep 10;43(3):135-142. doi: 10.4274/tpd.galenos.2019.6294.
The aim of the present study was to show the morphologic and molecular characterization of in grey mullet () caught from the Black Sea coasts of Turkey.
A total of 104 grey mullets caught from Turkish Black Sea coasts were obtained from fish markets between November 2017 and February 2018 and were examined for the presence of by using morphological and advanced molecular diagnostic methods.
Totally, three out of 104 fish specimens were found to be infected with large and white coloured plasmodia and the prevalence was determined as 2.8%. The myxospores were morphologically identified as , confirmed by using sequence analyses of their 18S rRNA gene regions. The shape of myxospores was oval and they were 8.6 (7.8-9.4) μm in length, 6.7 (6.1-7.3) μm in width and 4.7 (4.1-5.3) μm in thickness. Measuremenst of two pyriform polar capsules were 4.2 (3.7-4.6) μm in length ve 2.2 (2.1-2.4) μ m in width. The length of polar filament was determined as 27-51 μm. Nucleotide sequences of the 18S rRNA gene of the ES-2018-ERU isolate (MK012069) found in our study showed 99.8% and 100% similarity with isolates submitted to GenBank from Tunisia (AY129312) and South Korea (JF810537; KC733437), respectively. Pairwise genetic distance (%) between ES-2018-ERU isolate and the other which were identified in mullets from various countries were determined as 9.9-14.8%. Conclusion: This study provides first molecular
This study provides first molecular data on found in the scale of the grey mullets () from Turkey.
本研究旨在展示从土耳其黑海沿岸捕获的灰鲻（）的形态学和分子特征。
2017年11月至2018年2月期间，从土耳其黑海沿岸的鱼市获取了总共104条灰鲻，并使用形态学和先进的分子诊断方法检查是否存在。
总共发现104条鱼标本中有3条感染了大型白色疟原虫，感染率确定为2.8%。通过对其18S rRNA基因区域进行序列分析，在形态学上鉴定出粘孢子为。粘孢子的形状为椭圆形，长度为8.6（7.8 - 9.4）μm，宽度为6.7（6.1 - 7.3）μm，厚度为4.7（4.1 - 5.3）μm。两个梨形极囊的测量值为长度4.2（3.7 - 4.6）μm，宽度2.2（2.1 - 2.4）μm。极丝长度确定为27 - 51μm。在我们的研究中发现的ES - 2018 - ERU分离株（MK012069）的18S rRNA基因的核苷酸序列与从突尼斯（AY129312）和韩国（JF810537；KC733437）提交到GenBank的分离株分别显示出99.8%和100%的相似性。ES - 2018 - ERU分离株与在来自不同国家的鲻鱼中鉴定出的其他之间的成对遗传距离（%）确定为9.9 - 14.8%。结论：本研究提供了关于在土耳其灰鲻（）范围内发现的的首个分子数据。
本研究提供了关于在土耳其灰鲻（）范围内发现的的首个分子数据。
