Center for Scientific Research, San Pietro Hospital, Fatebenefratelli, Via Nomentana 261, 00161 Rome, Italy.
Am J Obstet Gynecol. 2010 Feb;202(2):196.e1-6. doi: 10.1016/j.ajog.2009.10.870. Epub 2009 Dec 24.
Plasma adrenomedullin (AM) concentrations are increased in fetal and maternal circulation in response to exogenous glucocorticoids administration. The role of corticosteroids and progesterone in regulating AM synthesis and secretion was investigated in amnion and chorion trophoblast cells of the fetal membranes and in placental trophoblast cells.
Cells were treated with betamethasone, hydrocortisone, and progesterone. Changes in AM output were measured with radioimmunoassay. Protein expression was evaluated with Western blot and immunohistochemistry.
Betamethasone stimulated AM secretion and protein expression in placental trophoblast cells and in amnion cells of the fetal membranes. Hydrocortisone and progesterone did not induce any effect either on secretion or protein expression in placenta and fetal membranes cells.
Glucocorticoids regulate AM secretion and expression by human placenta thereby promoting increased AM concentration in maternal and fetal circulation in circumstances characterized by increased cortisol levels.
在胎儿和母体循环中，血浆肾上腺髓质素 (AM) 浓度会因外源性糖皮质激素的给药而增加。本研究旨在探讨皮质类固醇和孕激素在调节羊膜和绒毛膜滋养层细胞及胎盘滋养层细胞中 AM 合成和分泌中的作用。
用倍他米松、氢化可的松和孕酮处理细胞。用放射免疫法测定 AM 的分泌变化。用 Western blot 和免疫组化评估蛋白表达。
倍他米松刺激胎盘滋养层细胞和羊膜细胞中 AM 的分泌和蛋白表达。氢化可的松和孕酮对胎盘和胎膜细胞的分泌或蛋白表达均无影响。
糖皮质激素通过人胎盘调节 AM 的分泌和表达，从而在皮质醇水平升高的情况下促进母胎循环中 AM 浓度的增加。
