Department of Cell and Organism Biology, Lund University, Helgonavägen 3b, SE-22362 Lund, Sweden.
Exp Cell Res. 2010 Mar 10;316(5):688-94. doi: 10.1016/j.yexcr.2009.12.016. Epub 2009 Dec 28.
The phenomenon of contact guidance on thin fibers has been known since the beginning of the 20th century when Harrison studied cells growing on fibers from spider's web. Since then many studies have been performed on structured surfaces and fibers. Here we present a new way to induce guidance of cells or cell processes using magnetic nanowires. We have manufactured magnetic Ni-nanowires (200 nm in diameter and 40 microm long) with a template-based electro-deposition method. Drops of a nanowire/ethanol suspension were placed on glass cover slips. The nanowires were aligned in an external magnetic field and adhered to the cover slips after evaporation of the ethanol. When the wires had adhered, the magnetic field was removed. L929 fibroblasts and dissociated dorsal root ganglia (DRG) neurons from mice were cultured on the nanowire-coated cover slips for 24 h and 72 h respectively. The fibroblasts were affected by the aligned nanowires and displayed contact guidance. Regenerated axons also displayed contact guidance on the wires. There were no overt signs of toxicity caused by Ni-wires. Aligned magnetic nanowires can be useful for lab-on-a-chip devices and medical nerve grafts.
自 20 世纪初 Harrison 研究蜘蛛丝纤维上生长的细胞以来，人们就已经知道了在细纤维上的接触引导现象。从那时起，许多关于结构化表面和纤维的研究已经完成。在这里，我们提出了一种使用磁性纳米线诱导细胞或细胞过程导向的新方法。我们使用基于模板的电沉积方法制造了直径为 200nm 且长 40μm 的磁性 Ni 纳米线。将纳米线/乙醇悬浮液滴放置在玻璃盖玻片上。在乙醇蒸发后，将纳米线在外磁场中对齐并粘附在盖玻片上。当纳米线粘附后，去除磁场。将 L929 成纤维细胞和从小鼠分离的背根神经节（DRG）神经元分别在纳米线涂层盖玻片上培养 24 小时和 72 小时。成纤维细胞受到对齐的纳米线的影响，并显示出接触引导。再生轴突也在导线上显示出接触引导。镍线没有明显的毒性迹象。对齐的磁性纳米线可用于芯片实验室设备和医学神经移植物。
