Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Minas Gerais, Brazil.
Transpl Immunol. 2010 Feb;22(3-4):195-7. doi: 10.1016/j.trim.2009.12.002. Epub 2009 Dec 24.
The high incidence of overwhelming postsplenectomy infection caused by Streptococcus pneumoniae can be reduced by splenic autotransplantation. In this study the effect of splenectomy and splenic autotransplantation on the immune response to S. pneumoniae infection was investigated. Balb/c mice were divided into three groups: splenectomized (SP), splenectomized and autotransplanted (AT), and sham operated control (CT). Five days post-infection the serum antibody levels were measured and the number of S. pneumoniae CFU, neutrophil accumulation and IL-17 production in the liver and lungs were investigated. SP mice showed greater number of bacteria in both organs and lower serum levels of S. pneumoniae-specific IgM, IgG1 and IgG2a antibodies. IL-17 production and neutrophil recruitment to the liver and lungs were lower in SP mice, in comparison with both the CT and the AT groups. Levels of S. pneumoniae-specific IgM, CFU counts, neutrophil accumulation and IL-17 production did not differ significantly between the CT and AT groups. These results suggest that splenic autotransplantation restores the capacity of splenectomized mice to fight S. pneumoniae infection.
脾切除术后由肺炎链球菌引起的感染发生率很高，可以通过脾自体移植来降低。本研究旨在探讨脾切除和脾自体移植对肺炎链球菌感染免疫反应的影响。将 Balb/c 小鼠分为三组：脾切除术（SP）组、脾切除术加自体移植（AT）组和假手术对照组（CT）组。感染后 5 天，检测血清抗体水平，并检测肺炎链球菌 CFU、肝脏和肺部中性粒细胞聚集和 IL-17 产生的数量。SP 小鼠在两个器官中均有更多的细菌，肺炎链球菌特异性 IgM、IgG1 和 IgG2a 抗体的血清水平较低。与 CT 和 AT 组相比，SP 小鼠的 IL-17 产生和中性粒细胞向肝脏和肺部的募集减少。CT 和 AT 组之间肺炎链球菌特异性 IgM、CFU 计数、中性粒细胞聚集和 IL-17 产生水平无显著差异。这些结果表明，脾自体移植恢复了脾切除小鼠对抗肺炎链球菌感染的能力。
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