Izbicki J R, Ziegler-Heitbrock H W, Raedler C, Kastl S, Bauhuber W, Wilker D K, Lütticken R, Ruckdeschel G, Schlunck T, Anke A
Department of Surgery, University of Munich, F.R.G.
J Trauma. 1991 Feb;31(2):189-95.
The existence of the overwhelming postsplenectomy infection syndrome in adults after traumatic splenectomy is controversial. Due to the similarity of the porcine immune system to man we chose the pig to study subsets of peripheral mononuclear cells after splenectomy and resistance to experimental Pneumococcal infection after splenic surgery and specific immunization. Female miniature pigs were assigned to four operative groups: sham operation, splenectomy, splenic resection, and heterotopic splenic autotransplantation. Hematologic and flow cytometric analysis of mononuclear cells and their subsets revealed a marked leukocytosis following splenectomy and autotransplantation but no significant shift in monocyte and B-cell numbers. Response of leukocytes to septicemia, bacterial elimination from peripheral blood, and mortality were not affected by splenectomy or spleen-preserving operations. Mortality of splenectomized animals was 18%, compared to 42% in sham-operated controls (difference not significant). Immunization protected animals from development of leukopenia, and led to an enhanced bacterial elimination, and a significantly decreased mortality of 5%, compared to 48% in nonimmune animals. Thus our data do not show significant effects of splenectomy on subsets of porcine mononuclear cells or on resistance to experimental Pneumococcal septicemia.
外伤性脾切除术后成人中压倒性的脾切除后感染综合征的存在存在争议。由于猪的免疫系统与人相似，我们选择猪来研究脾切除术后外周单核细胞亚群以及脾切除和特异性免疫后对实验性肺炎球菌感染的抵抗力。将雌性小型猪分为四个手术组：假手术、脾切除术、脾切除加脾细胞移植和异位脾自体移植。对单核细胞及其亚群进行血液学和流式细胞术分析发现，脾切除术后和自体移植后出现明显的白细胞增多，但单核细胞和B细胞数量无显著变化。脾切除术或保脾手术对白细胞对败血症的反应、外周血细菌清除及死亡率均无影响。脾切除动物的死亡率为18%，假手术对照组为42%（差异不显著）。免疫可保护动物不发生白细胞减少，并导致细菌清除增强，死亡率显著降低至5%，而非免疫动物为48%。因此，我们的数据未显示脾切除术对猪单核细胞亚群或对实验性肺炎球菌败血症抵抗力有显著影响。
