Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Vet J. 2011 Feb;187(2):279-81. doi: 10.1016/j.tvjl.2009.11.020. Epub 2009 Dec 29.
Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM+), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA+). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA+ samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 of 19 EM+/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples.
对 476 份腹泻仔猪粪便样本进行了电子显微镜（EM）和基于 A 组轮状病毒 VP6 蛋白的单克隆抗体的竞争阻断（CB）-ELISA 分析。EM 和/或 CB-ELISA 在 111 份（23.3%）样本中检测到轮状病毒。其中，通过 EM（EM+）在 83 份（74.8%）样本中鉴定出所有轮状病毒群，而通过 CB-ELISA（ELISA+）在 90 份（81.1%）样本中鉴定出 A 组轮状病毒。然而，只有 62 份（55.9%）样本通过两种检测方法均呈阳性。28 份（25.2%）EM-/CB-ELISA+样本的发现说明了 CB-ELISA 方法的高灵敏度。通过 PCR 分析，在 19 份 EM+/CB-ELISA-样本中分别发现了 B 组和 C 组轮状病毒。尽管该研究表明 CB-ELISA 在轮状病毒检测中具有较高的灵敏度，但研究结果强调需要在临床样本中使用一系列诊断方法来检测这些病毒。
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