Department of Sericulture Science, South China Agricultural University, Guangzhou 510642, China.
J Invertebr Pathol. 2010 Jun;104(2):134-9. doi: 10.1016/j.jip.2009.11.007. Epub 2009 Dec 29.
Biological tests demonstrated that the inactivation of Nosema bombycis (N. bombycis) spores by chlorine dioxide (ClO(2)) occurs very fast and is highly sensitive. The lowest effective inactivation dosage and time was 15mg/mL for 30min. The inactivation of spores was additionally verified by using double color fluorescence stain and spore germination testing. A series of biological changes, including a large number of substrates that were leaked out from the spores included proteins, DNA, polysaccharide, K(+), and Ca(2+), occurred a short time after N. bombycis spores were treated with ClO(2). In addition, the lipid of spores was disrupted and ATPase activity was inhibited, which resulted in the destruction of the inner structure of the spores.
生物测试表明，二氧化氯（ClO₂）对家蚕微孢子虫（N. bombycis）孢子的失活作用非常迅速且高度敏感。最低有效失活剂量和时间为 15mg/mL 作用 30min。通过双色荧光染色和孢子发芽试验进一步验证了孢子的失活。在 N. bombycis 孢子用 ClO₂处理后很短的时间内，就发生了一系列的生物学变化，包括大量从孢子中泄漏出来的基质，包括蛋白质、DNA、多糖、K⁺和 Ca²⁺。此外，孢子的脂质被破坏，ATP 酶活性受到抑制，导致孢子内部结构的破坏。
