Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, PR China.
Fish Shellfish Immunol. 2010 Apr;28(4):549-54. doi: 10.1016/j.fsi.2009.12.010. Epub 2009 Dec 30.
The p8 is a transcription factor with a basic helix-loop-helix motif and a nuclear localization signal. A zebrafish p8 cDNA, which consists of 732 bp and encodes 75 amino acids, was identified in this paper. Sequence alignment showed that the bHLH region of p8 was well-conserved during the evolution. Phylogenetic analysis revealed that zebrafish p8 was close to its homologous protein in frog, together clustering to the clade of vertebrates. The zebrafish p8 mRNA expression levels varied much among the detected adult tissues, with the obvious higher expression in backbone and liver. During embryogenesis, the expression of zebrafish p8 mRNA was in higher levels in cleavage stage, decreased from blastula to segmentation stage, but sharply elevated at hatching stage. Quantitative real-time PCR assay suggested up-regulation expressions of zebrafish p8 on a wide range of cellular stressors such as starvation, temperature, osmotic pressure and pH value, implying an important role of p8 gene in response to stress.
p8 是一种具有碱性螺旋-环-螺旋基序和核定位信号的转录因子。本文鉴定了一个包含 732 个碱基对、编码 75 个氨基酸的斑马鱼 p8 cDNA。序列比对表明，p8 的 bHLH 区域在进化过程中高度保守。系统发生分析显示，斑马鱼 p8 与其在青蛙中的同源蛋白密切相关，共同聚类到脊椎动物的分支。斑马鱼 p8 mRNA 在检测到的成年组织中的表达水平差异很大，在脊柱和肝脏中表达水平明显较高。在胚胎发生过程中，斑马鱼 p8 mRNA 的表达水平在卵裂期较高，从囊胚期到分节期降低，但在孵化期急剧升高。定量实时 PCR 检测表明，p8 基因在广泛的细胞应激条件下（如饥饿、温度、渗透压和 pH 值）表达上调，这表明 p8 基因在应对应激方面发挥着重要作用。
