Krens S F Gabby, He Shuning, Spaink Herman P, Snaar-Jagalska B Ewa
Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
Gene Expr Patterns. 2006 Oct;6(8):1019-26. doi: 10.1016/j.modgep.2006.04.008. Epub 2006 May 8.
The mitogen activated protein kinases (MAPK) family pathway is conserved in evolution through the plant and animal kingdoms. These proteins have been implicated in diverse cellular processes including cell growth, proliferation, differentiation, survival and development. In this study we annotated and cloned members of the zebrafish MAPK gene-family, containing the ERK, JNK and p38 subfamilies. Their sequences were compared to orthologs of other vertebrates (human, mouse and rat) and the temporal and spatial expression levels of the zebrafish mapk genes were determined during early zebrafish development. Semi-quantitative reverse transcriptase-PCR analysis revealed that most mapk genes are expressed throughout zebrafish development. Erk2,3 and p38a were expressed at a constant level throughout zebrafish embryogenesis, whereas erk1,4,5,6,7 and p38b showed specific temporal expression patterns. The spatial expression patterns were obtained by whole mount in situ hybridization at 24 h post fertilization (hpf) and 48 hpf embryos. The expression patterns were localized in specific regions at both stages and were tightly regulated during embryogenesis. For p38b, no staining was detected at 24 and 48 hpf. However, its expression was demonstrated at blastula-stage. Together, we identified the zebrafish orthologs of the zebrafish MAPK gene family and determined their specific spatial and temporal expression and distribution patterns during zebrafish embryogenesis.
丝裂原活化蛋白激酶（MAPK）家族通路在植物和动物界的进化过程中是保守的。这些蛋白质参与了多种细胞过程，包括细胞生长、增殖、分化、存活和发育。在本研究中，我们注释并克隆了斑马鱼MAPK基因家族的成员，该家族包含ERK、JNK和p38亚家族。将它们的序列与其他脊椎动物（人类、小鼠和大鼠）的直系同源物进行比较，并确定斑马鱼mapk基因在斑马鱼早期发育过程中的时空表达水平。半定量逆转录聚合酶链反应分析表明，大多数mapk基因在斑马鱼整个发育过程中都有表达。Erk2、3和p38a在斑马鱼胚胎发育过程中表达水平恒定，而erk1、4、5、6、7和p38b则表现出特定的时间表达模式。通过对受精后24小时（hpf）和48 hpf胚胎进行全胚胎原位杂交获得空间表达模式。在这两个阶段，表达模式都定位于特定区域，并且在胚胎发育过程中受到严格调控。对于p38b，在24和48 hpf时未检测到染色。然而，其在囊胚期有表达。我们共同鉴定了斑马鱼MAPK基因家族的斑马鱼直系同源物，并确定了它们在斑马鱼胚胎发育过程中的特定时空表达和分布模式。
