Department of Molecular Biology and Biotechnology, Atomic Energy Commission, PO Box 6091, Damascus, Syria.
Vaccine. 2010 Feb 17;28(7):1766-70. doi: 10.1016/j.vaccine.2009.12.012. Epub 2009 Dec 28.
The periplasmic binding protein (P39) antigen of Brucella melitensis 16 M was previously identified as Th1 dominant antigens. In this study, the potential for this antigen to function as vaccine against B. melitensis 16 M infection in BALB/c mice has been analyzed, and the humoral and cellular immune responses induced have been also characterized. Mice were injected intraperitoneally with live Escherichia coli alone or with that which express Brucella P39, two times at 4 weeks intervals. The live E. coli BL21 (DE3) pEt15b-p39 vaccine elicited a T-cell-proliferative response and also induced a gamma interferon production upon re-stimulation with either the bacterial extract or P39 as a specific antigen. Also the live E. coli BL21 (DE3) pEt15b-p39 vaccine has been found to induce a strong humoral response (IgG1 and IgG2a). Compared to the saline-inoculated control, vaccination of mice with E. coli BL21pEt15b-p39 at 3 weeks prior to the challenge infection, significantly reduced the number of strain 16 M bacteria in spleens at 4 and 8 weeks post-challenge infection in all vaccinated mice (p<0.001).
布氏杆菌 melitensis 16 M 的周质结合蛋白（P39）抗原先前被鉴定为 Th1 优势抗原。在这项研究中，分析了这种抗原作为针对 B. melitensis 16 M 感染的疫苗的潜力，并对其诱导的体液和细胞免疫反应进行了特征描述。将活大肠杆菌单独或表达布鲁氏菌 P39 的大肠杆菌注入 BALB/c 小鼠的腹腔中，每 4 周注射两次。活大肠杆菌 BL21（DE3）pEt15b-p39 疫苗引起 T 细胞增殖反应，并在再次刺激细菌提取物或 P39 作为特异性抗原时诱导产生伽马干扰素。活大肠杆菌 BL21（DE3）pEt15b-p39 疫苗还被发现诱导强烈的体液反应（IgG1 和 IgG2a）。与生理盐水接种对照相比，在挑战感染前 3 周用 E. coli BL21pEt15b-p39 接种疫苗可显著减少所有接种疫苗小鼠在 4 周和 8 周攻毒后脾脏中 16 M 菌株的数量（p<0.001）。
