Amani Jafar, Ghasemi Amir, Ranjbar Reza, Shabani Mahdi, Zandemami Mahdi, Golmohammadi Reza
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2015 Apr;18(4):350-5.
Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4(+) and CD8(+) T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates.
A chimeric gene encoding trigger factor (TF), Omp31(48-74) and BP26(87-111) fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coli BL21-DE3 and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits.
Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in 76.5% of sera obtained from infected patients.
These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies.
布鲁氏菌属是兼性细胞内病原体，可在专职和非专职吞噬细胞中存活并繁殖。针对羊种布鲁氏菌的免疫涉及抗原特异性CD4(+)和CD8(+) T细胞活化及体液免疫反应。由于减毒活疫苗存在负面问题，人们已将大量注意力集中在寻找布鲁氏菌保护性抗原，以将其作为潜在的亚单位疫苗候选物。
成功克隆了编码来自羊种布鲁氏菌的触发因子（TF）、Omp31(48 - 74)和BP26(87 - 111)片段（TOB）的嵌合基因，在大肠杆菌BL21 - DE3中表达，并通过Ni - NTA琼脂糖柱纯化。已在布鲁氏菌感染的人血清和由羊种布鲁氏菌疫苗接种兔制备的混合血清中研究了针对重组TOB（rTOB）的抗体。
我们的结果表明，免疫的兔混合血清与rTOB强烈反应。此外，在76.5%的感染患者血清中可检测到针对rTOB的抗体。
这些发现表明，rTOB可能提供一种潜在的免疫原性候选物，可在未来疫苗研究中予以考虑。
