微阵列探针和探针组
Microarray probes and probe sets.
作者信息
Liu Hongfang, Bebu Ionut, Li Xin
机构信息
Department of Biostatistics, Georgetown University Medical Center, Washington, DC 20007, USA.
出版信息
Front Biosci (Elite Ed). 2010 Jan 1;2(1):325-38. doi: 10.2741/e93.
DNA microarrays have gained wide use in biomedical research by simultaneously monitoring the expression levels of a large number of genes. The successful implementation of DNA microarray technologies requires the development of methods and techniques for the fabrication of microarrays, the selection of probes to represent genes, the quantification of hybridization, and data analysis. In this paper, we concentrate on probes that are either spotted or synthesized on the glass slides through several aspects: sources of probes, the criteria for selecting probes, tools available for probe selections, and probes used in commercial microarray chips. We then provide a detailed review of one type of DNA microarray: Affymetrix GeneChips, discuss the need to re-annotate probes, review different methods for regrouping probes into probe sets, and compare various redefinitions through public available datasets.
DNA微阵列通过同时监测大量基因的表达水平，在生物医学研究中得到了广泛应用。DNA微阵列技术的成功实施需要开发用于微阵列制造的方法和技术、代表基因的探针选择、杂交定量以及数据分析。在本文中，我们从几个方面集中讨论在玻璃载玻片上点样或合成的探针：探针来源、探针选择标准、可用于探针选择的工具以及商业微阵列芯片中使用的探针。然后，我们详细综述了一种DNA微阵列：Affymetrix基因芯片，讨论了重新注释探针的必要性，综述了将探针重新分组为探针集的不同方法，并通过公开可用数据集比较了各种重新定义。
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