The Research Institute of the University of Bucharest, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.
Department of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania.
Int J Mol Sci. 2022 Jun 10;23(12):6499. doi: 10.3390/ijms23126499.
(the fruit fly) is a valuable experimental platform for modeling host-pathogen interactions. It is also commonly used to define innate immunity pathways and to understand the mechanisms of both host tolerance to commensal microbiota and response to pathogenic agents. Herein, we investigate how the host response to bacterial infection is mirrored in the expression of genes of Imd and Toll pathways when strains with different genetic backgrounds are infected with ATCC 27853. Using microarray technology, we have interrogated the whole-body transcriptome of infected versus uninfected fruit fly males with three specific genotypes, namely wild-type Oregon, /TM6B and /. While the expression of genes pertaining to Imd and Toll is not significantly modulated by infection in Oregon males, many of the components of these cascades are up- or downregulated in both infected and uninfected /TM6B and / males. Thus, our results suggest that a genetic background modulates the gene expression profiles of Imd and Toll cascades involved in the innate immune response of inducing the occurrence of immunological dysfunctions in mutants.
（果蝇）是一种用于模拟宿主-病原体相互作用的有价值的实验平台。它也常用于定义先天免疫途径，并了解宿主对共生微生物的耐受性和对病原体的反应的机制。在此，我们研究了当具有不同遗传背景的菌株感染 ATCC 27853 时，Imd 和 Toll 途径的基因表达如何反映宿主对细菌感染的反应。使用微阵列技术，我们检测了三种特定基因型（野生型俄勒冈、TM6B 和 ）感染与未感染雄性果蝇的全基因组转录组。虽然 Imd 和 Toll 相关基因的表达在俄勒冈雄性果蝇的感染中没有显著调节，但这些级联的许多成分在感染和未感染的 TM6B 和 雄性果蝇中均上调或下调。因此，我们的结果表明，遗传背景调节了 Imd 和 Toll 级联参与先天免疫反应的基因表达谱，导致 突变体发生免疫功能障碍。
