Wang Yu-Hua, Yang Hong-Qin, Xie Shu-Sen, Ye Zhen, Su Yi-Ming
Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Lab of Photonic Technology, Institute of Laser and OptoElectronics Technology, Fujian Normal University, Fuzhou 350007, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Oct;29(10):2751-5.
Spectral characteristics of normal female breast samples in the 350-850 nm wavelength range were measured using a UV/Vis/NIR spectrophotometer system with integrating sphere attachment for measuring the diffuse reflectance and transmittance. The optical properties of normal breast tissue in vitro were obtained by the inverse adding doubling method. And then the optical penetration depths in this spectral range were analyzed based on the principle of tissue optics. The results show that the reduced scattering coefficient of normal female breast tissue is significantly higher than the absorption coefficient in the 350-850 nm wavelength range. The reduced scattering coefficient decreases with the wavelength increment. It reaches maximum at shorter wavelengths with a decrease at longer wavelengths and ranges from 9.731 mm(-1) at 350 nm to 1.476 mm(-1) at 850 nm. The absorption coefficient of normal breast tissue is about from 0.798 mm(-1) at 350 nm to 0.102 mm(-1) at 850 nm. The maximal and minimal values are at 350 nm and 850 nm respectively. An absorption peak for the normal breast tissue is at 410 nm of wavelength with the value of 0.506 mm(-1), which belongs to hemoglobin. The absorption coefficient remains relatively constant when the wavelength is longer than 600 nm. The optical penetration depth increases with the wavelength increment and ranges about from 0.199 mm at 350 nm to 1.439 mm at 850 nm. Deep penetration depth noted in normal breast samples, especially at longer wavelengths, reflects the weak absorption and reduced scattering at these wavelengths. The calculated optical parameters of normal breast samples by the inverse adding doubling method agree well with the Monte Carlo simulations. This study may be useful for breast optical biopsy or the optical diagnosis of breast diseases.
使用配备积分球附件以测量漫反射率和透射率的紫外/可见/近红外分光光度计系统，测量了350 - 850纳米波长范围内正常女性乳腺样本的光谱特性。通过逆倍增法获得了体外正常乳腺组织的光学特性。然后基于组织光学原理分析了该光谱范围内的光穿透深度。结果表明，在350 - 850纳米波长范围内，正常女性乳腺组织的约化散射系数显著高于吸收系数。约化散射系数随波长增加而降低。它在较短波长处达到最大值，在较长波长处减小，范围从350纳米处的9.731毫米⁻¹到850纳米处的1.476毫米⁻¹。正常乳腺组织的吸收系数约从350纳米处的0.798毫米⁻¹到850纳米处的0.102毫米⁻¹。最大值和最小值分别在350纳米和850纳米处。正常乳腺组织的一个吸收峰在波长410纳米处，值为0.506毫米⁻¹，属于血红蛋白。当波长大于600纳米时，吸收系数保持相对恒定。光穿透深度随波长增加而增加，范围约从350纳米处的0.199毫米到850纳米处的1.439毫米。在正常乳腺样本中观察到的深穿透深度，特别是在较长波长处，反映了这些波长处的弱吸收和减少的散射。通过逆倍增法计算的正常乳腺样本的光学参数与蒙特卡罗模拟结果吻合良好。本研究可能对乳腺光学活检或乳腺疾病的光学诊断有用。
