Krett G, Palatinszky M
Department of Microbiology, Eötvös Loránd University, Budapest, Hungary.
Acta Microbiol Immunol Hung. 2009 Dec;56(4):339-55. doi: 10.1556/AMicr.56.2009.4.4.
Lake Hévíz is the largest natural thermal lake of Europe, harboring special bacterial communities. The aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on Actinobacteria, by using cultivation-based and -independent molecular methods. Samples from two depths were taken in two different locations in October 2007. 245 strains were isolated, grouped to 85 OTUs by ARDRA, and identified by 16S rDNA sequencing. Most of the strains showed highest sequence similarity with Bacillus and related genera. Strains belonging to the phylum Actinobacteria were identified as members of Arthrobacter, Brachybacterium, Brevibacterium, Curtobacterium, Friedmanniella, Gordonia, Kocuria, Microbacterium, Micrococcus, Micromonospora, Mycobacterium, Rhodococcus, Streptomyces and Williamsia . Two clone libraries were constructed from H3M and H4M samples, providing 288 and 192 clones which were grouped to 150 and 125 OTUs, respectively, by ARDRA. The two most abundant group of the H4M library were OP8-related. The phylum Proteobacteria was represented mostly by delta-Proteobacteria, other relevant groups were Cyanobacteria, Bacteroidetes, Acidobacteria and beta-Proteobacteria. The H3M library was dominated by Cyanobacteria, Verrucomicrobia, beta-Proteobacteria, gamma-Proteobacteria and delta-Proteobacteria. Chloroflexi, Bacteroidetes, Spirochetes and Firmicutes were scarce. Results from the clone libraries were compared to the length-heterogeneity-PCR fingerprints of the communities.
赫维兹湖是欧洲最大的天然温泉湖，拥有特殊的细菌群落。本研究的目的是通过基于培养和非培养的分子方法，获取有关沉积物微生物群的分布和物种多样性的信息，特别关注放线菌。2007年10月在两个不同地点采集了两个深度的样本。分离出245株菌株，通过ARDRA将其分为85个OTU，并通过16S rDNA测序进行鉴定。大多数菌株与芽孢杆菌及相关属显示出最高的序列相似性。属于放线菌门的菌株被鉴定为节杆菌属、短杆菌属、短棒杆菌属、短小杆菌属、弗里德菌属、戈登氏菌属、考克氏菌属、微杆菌属、微球菌属、小单孢菌属、分枝杆菌属、红球菌属、链霉菌属和威廉姆斯菌属的成员。从H3M和H4M样本构建了两个克隆文库，分别提供了288个和192个克隆，通过ARDRA将其分别分为150个和125个OTU。H4M文库中两个最丰富的类群与OP8相关。变形菌门主要由δ-变形菌代表，其他相关类群为蓝细菌、拟杆菌门、酸杆菌门和β-变形菌。H3M文库以蓝细菌、疣微菌门、β-变形菌、γ-变形菌和δ-变形菌为主。绿弯菌门、拟杆菌门、螺旋体门和厚壁菌门较少。将克隆文库的结果与群落的长度异质性PCR指纹图谱进行了比较。
