Laubscher T A, Harris N, Chalton D O
Department of Dermatology, University of Cape Town/Groote Schuur Hospital, South Africa.
Br J Dermatol. 1991 Feb;124(2):163-7. doi: 10.1111/j.1365-2133.1991.tb00426.x.
Serum was obtained from 21 normal and 22 psoriatic subjects, and the severity of skin disease in the psoriatic patients was recorded using the PASI score. The scores ranged from 1.8 to 51.0. The growth stimulatory effect of the sera on normal dermal fibroblasts in cell culture was assessed by measuring [3H]-thymidine uptake. Each serum was assessed at four concentrations (2,5, 10,20%). The psoriatic sera were more growth stimulatory than normal sera, but this difference was statistically significant (P less than 0.02) only at 20% serum concentration. Eleven of the 22 psoriatic serum samples had a mitogenic effect greater than the mean +/- SEM of all the normal sera; these sera were then from patients with PASI scores of 4.5-51.0. Six of these psoriatic subjects were recalled after 5 months; their PASI scores were reassessed, and the mitogenic effect of new serum samples was compared with that of the initial samples. All of these patients displayed a change in serum mitogenic effect, but this was not consistent with the change in severity of skin disease over the corresponding time period. In one subject, the severity of the psoriasis had increased marginally over the 5 months, while the mitogenic effect of her serum decreased significantly (mean counts of 43808 vs. 32660; P = 0.0029).
从21名正常受试者和22名银屑病患者身上采集了血清，使用银屑病面积和严重程度指数（PASI）对银屑病患者的皮肤病严重程度进行记录。分数范围为1.8至51.0。通过测量[3H] - 胸腺嘧啶核苷摄取来评估血清在细胞培养中对正常真皮成纤维细胞的生长刺激作用。每种血清在四个浓度（2%、5%、10%、20%）下进行评估。银屑病血清比正常血清具有更强的生长刺激作用，但仅在20%血清浓度时，这种差异具有统计学意义（P小于0.02）。22份银屑病血清样本中有11份的促有丝分裂作用大于所有正常血清的平均值±标准误；这些血清来自PASI评分为4.5 - 51.0的患者。5个月后召回了其中6名银屑病患者；重新评估他们的PASI评分，并将新血清样本的促有丝分裂作用与初始样本进行比较。所有这些患者的血清促有丝分裂作用都发生了变化，但这与相应时间段内皮肤病严重程度的变化不一致。在一名患者中，银屑病的严重程度在5个月内略有增加，而她血清的促有丝分裂作用却显著下降（平均计数分别为43808和32660；P = 0.0029）。
