Benetti Ana Helena, Schein Fábio Bernardo, dos Santos Thaís Rabelo, Toniollo Gilson Hélio, da Costa Alvimar José, Mineo José Roberto, Lobato Janaína, de Oliveira Silva Deise Aparecida, Gennari Solange Maria
Faculdade de Medicina Veterinária, Universidade de Cuiabá-UNIC, Av. Beira Rio 3100, Jardim Europa, CEP 78015-480 Cuiabá-MT, Brazil.
Rev Bras Parasitol Vet. 2009 Dec;18 Suppl 1:29-33. doi: 10.4322/rbpv.018e1005.
Considering the importance of neosporosis in the animal health and production, the frequency of antibodies to Neospora caninum was evaluated in dairy cattle of the Southwestern region of Mato Grosso State, Brazil, in addition to serum samples obtained from dogs and humans living in the farms. A total of 1036 serum samples were analyzed, from which 932 were from dairy bovine females, 37 from dogs and 67 from humans, from 24 farms and examined by the indirect fluorescent antibody test (IFAT). Reactive human serum samples were retested by Western- blotting to confirm the results. Antibodies to N. caninum were found in 499 cattle sera (53.5%), with at least one positive in each farm, 25 dog sera (67.6%) and seven human sera (10.5%). There was no significant difference in the number of positive cattle sera according to age group. The results indicate a wide dissemination of N. caninum in the studied region.
鉴于新孢子虫病对动物健康和生产的重要性，除了从生活在农场的狗和人类身上采集的血清样本外，还对巴西马托格罗索州西南部地区的奶牛进行了犬新孢子虫抗体检测。共分析了1036份血清样本，其中932份来自奶牛雌性，37份来自狗，67份来自人类，这些样本来自24个农场，并通过间接荧光抗体试验（IFAT）进行检测。对反应性人类血清样本进行了蛋白质印迹法复检以确认结果。在499份牛血清（53.5%）中发现了犬新孢子虫抗体，每个农场至少有一份阳性，25份狗血清（67.6%）和7份人类血清（10.5%）中也发现了抗体。根据年龄组，阳性牛血清数量没有显著差异。结果表明犬新孢子虫在研究区域广泛传播。
