Gaxiola-Camacho Soila, García-Vázquez Zeferino, Cruz-Vázquez Carlos, Portillo-Loera Jesus, Vázquez-Peláez Carlos, Quintero-Martínez Maria Teresa, Rosario-Cruz Rodrigo
Instituto Tecnológico El Llano Aguascalientes, A.P. 74-2, Admon, Aguascalientes, Mexico.
Rev Bras Parasitol Vet. 2009 Oct-Dec;18(4):9-13. doi: 10.4322/rbpv.01804002.
The objective of this work was to study the reproductive behavior of two strains of R. (B.) microplus, one wild-caught (Native) and one reference strain (Media Joya) for two years, measuring the reproductive efficiency index (REI) and reproductive aptitude index (RAI). Engorged ticks from each strain were collected monthly from February 2002 to February 2004. These were weighed and kept in the laboratory until egg-laying. Oogenic masses were individually weighed and incubated until larvae emergence. REI and RAI were calculated from each sample, grouping ticks by weight in 100 mg range classes. An analysis of variance and Duncan's multiple rank tests were carried out on the data (P < 0.05). Pearson's correlation coefficients were estimated (P < 0.01) in order to determine the relationship between engorgement weight and the weight and number of laid and hatched eggs. The Native strain showed a higher engorgement weight than Media Joya strain. In both strains REI and RAI were similar in all weight classes; with a quadratic tendency in Media Joya and linear-quadratic in the Native strain (P < 0.05). Significant differences where observed when REI and RAI of both strains were compared along the duration of the study (P < 0.01) with the Native strain performing better in both indexes, including better performance during the dry season. All parameters studied showed highly significant correlation; eggs laid and eggs hatched were notably highly correlated, 0.94 and 0.91, for Media Joya strain and Native strain respectively.
本研究的目的是对两种微小牛蜱（R. (B.) microplus）菌株的繁殖行为进行为期两年的研究，其中一种是野生捕获的（本地菌株），另一种是参考菌株（梅迪亚·乔亚），测量繁殖效率指数（REI）和繁殖能力指数（RAI）。从2002年2月至2004年2月，每月收集每个菌株饱血蜱。对其进行称重，并保存在实验室直至产卵。对产卵团分别称重并孵化直至幼虫出现。根据每个样本计算REI和RAI，将蜱按100毫克范围的重量类别进行分组。对数据进行方差分析和邓肯多重秩检验（P < 0.05）。估计皮尔逊相关系数（P < 0.01），以确定饱血重量与产卵和孵化的卵的重量及数量之间的关系。本地菌株的饱血重量高于梅迪亚·乔亚菌株。在两个菌株中，所有重量类别的REI和RAI都相似；梅迪亚·乔亚菌株呈二次趋势，本地菌株呈线性 - 二次趋势（P < 0.05）。在研究期间比较两个菌株的REI和RAI时观察到显著差异（P < 0.01），本地菌株在两个指标上表现更好，包括在旱季表现更佳。所有研究参数均显示高度显著的相关性；梅迪亚·乔亚菌株和本地菌株产卵数与孵化卵数的相关性尤为显著，分别为0.94和0.91。
