Ma Miling, Chen Ze, Liu Aihong, Ren Qiaoyun, Liu Junlong, Liu Zhijie, Li Youquan, Yin Hong, Guan Guiquan, Luo Jianxun
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou, Gansu 730046, P. R. China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P. R. China.
Korean J Parasitol. 2016 Jun;54(3):301-5. doi: 10.3347/kjp.2016.54.3.301. Epub 2016 Jun 30.
In order to determine the effect of various hosts on feeding performance of Rhipicephalus (Boophilus) microplus, we used 3 mammalian species as hosts, cattle (Qinchuan), sheep (T an), and rabbits (Japanese white rabbit) for infest-ing ticks. Five hundreds of R. microplus larvae were exposed to each animal (3 animals/host species). Tick recoveries were 11.0%, 0.47%, and 5.5% from cattle, sheep, and rabbits, respectively. The averages of tick feeding periods were not significantly different on cattle, sheep, and rabbits, 28.8, 25.3, and 26.7 days, respectively. The average weights of individual engorged female from cattle, sheep, and rabbits were 312.5, 219.1, and 130.2 mg, respectively and those of egg mass weights each to 85.0, 96.6, and 17.8 mg. The highest egg hatching rate was in the ticks from cattle (96.0%), fol-lowed by those from rabbits (83.0%) and sheep (19.2%). These data suggest that rabbits could be as an alternative host to cultivate R. microplus for evaluating vaccines and chemical and biological medicines against the tick in the laboratory, although the biological parameters of ticks were less than those from cattle.
为了确定不同宿主对微小牛蜱（Rhipicephalus (Boophilus) microplus）取食性能的影响，我们使用了3种哺乳动物作为宿主，即牛（秦川牛）、绵羊（滩羊）和兔子（日本白兔）来饲养蜱虫。每种动物（每种宿主动物3只）暴露500只微小牛蜱幼虫。从牛、绵羊和兔子身上回收的蜱虫分别为11.0%、0.47%和5.5%。蜱虫在牛、绵羊和兔子身上的平均取食期无显著差异，分别为28.8天、25.3天和26.7天。从牛、绵羊和兔子身上采集的饱血雌蜱个体平均重量分别为312.5毫克、219.1毫克和130.2毫克，卵块重量分别为85.0毫克、96.6毫克和17.8毫克。最高的卵孵化率来自牛身上的蜱虫（96.0%），其次是兔子身上的蜱虫（83.0%）和绵羊身上的蜱虫（19.2%）。这些数据表明，兔子可作为一种替代宿主，用于在实验室中培育微小牛蜱，以评估针对该蜱虫的疫苗以及化学和生物药物，尽管蜱虫的生物学参数低于来自牛的蜱虫。
