Department of Infertility, Zekai Tahir Burak Women's Health Care Education and Research Hospital, Ankara, Turkey.
Arch Gynecol Obstet. 2011 Feb;283(2):267-72. doi: 10.1007/s00404-009-1344-1. Epub 2009 Dec 30.
Endometriosis is a chronic gynecological disease characterized by the growth of hormonally responsive, endometrial tissue outside the uterine cavity. The present study aims to analyze two vascular endothelial growth factor (VEGF) polymorphisms (-460 C/T and +405 C/G) in Turkish women with and without endometriosis.
A case-control study was undertaken at the Infertility Department of Zekai Tahir Burak Women's Health Care Education and Research Hospital. The single nucleotide polymorphisms, -460 C/T and +405 C/G, in the 5'-untranslated region of the VEGF gene were tested in 98 affected women and 94 women with no laparoscopic evidence of disease. Endometriosis was also confirmed histologically. Following genomic extraction of genomic DNA, genotyping of the -460 C/T and +405 C/G polymorphisms of the VEGF gene were performed by polymerase chain reaction and restriction fragment length polymorphism assay. Nominal data were evaluated by Pearson Chi-square or Fisher's Exact test, where applicable. Odds ratios and 95% confidence intervals were also calculated. A P value less than 0.05 was considered statistically significant.
Demographic data were similar among groups. The genotype and allele frequencies of the -460 C/T polymorphism did not differ significantly between cases and controls. In contrast, the genotype (P < 0.001) and allele frequencies (P < 0.001) of +405 C/G polymorphism showed a significant difference between cases and controls. Regardless of the early or advanced stage, women with endometriosis showed a higher incidence of the +405 GC genotype and +405G allele when compared with the controls.
These data suggest that VEGF +405 GC genotype and +405G allele may be associated with the risk of developing early and advanced stage endometriosis in the Turkish population.
子宫内膜异位症是一种慢性妇科疾病，其特征是在子宫腔外生长激素反应性的子宫内膜组织。本研究旨在分析土耳其患有和不患有子宫内膜异位症的女性中两种血管内皮生长因子 (VEGF) 多态性（-460C/T 和 +405C/G）。
这项病例对照研究在 Zekai Tahir Burak 妇女健康保健教育和研究医院的不孕不育科进行。在 98 名受影响的女性和 94 名没有腹腔镜疾病证据的女性中测试了 VEGF 基因 5'-非翻译区的单核苷酸多态性-460C/T 和 +405C/G。子宫内膜异位症也通过组织学证实。在提取基因组 DNA 后，通过聚合酶链反应和限制性片段长度多态性分析对 VEGF 基因的 -460C/T 和 +405C/G 多态性进行基因分型。适用时，通过皮尔逊卡方或 Fisher 精确检验评估名义数据。还计算了优势比和 95%置信区间。P 值小于 0.05 被认为具有统计学意义。
组间的人口统计学数据相似。-460C/T 多态性的基因型和等位基因频率在病例和对照组之间没有显著差异。相比之下，+405C/G 多态性的基因型（P<0.001）和等位基因频率（P<0.001）在病例和对照组之间存在显著差异。无论早期还是晚期，患有子宫内膜异位症的女性与对照组相比，+405GC 基因型和+405G 等位基因的发生率更高。
这些数据表明，VEGF +405GC 基因型和+405G 等位基因可能与土耳其人群中早期和晚期子宫内膜异位症的发病风险相关。
