Department of Obstetrics and Gynecology, Third Obstetrics and Gynecology, Sisli Etfal Training and Research Hospital, Istanbul, Turkey.
Int J Immunogenet. 2012 Jun;39(3):241-6. doi: 10.1111/j.1744-313X.2011.01082.x. Epub 2012 Jan 18.
We investigated the effect of polymorphism at +813 locus of vascular endothelial growth factor (VEGF) gene on predisposition to preterm labour and pre-eclampsia (PE). We examined polymorphism of the VEGF +813 gene of foetuses from umbilical cord blood in 31 cases of preterm labour, 34 pre-eclamptic and 58 healthy term labour. VEGF +813 gene polymorphisms were studied using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. In preterm group, foetal CC genotype was found at 80.6%, and CT genotype was seen at 19.4%. No any TT genotype was detected in preterm group. CC genotype of VEGF 813 gene was significantly more frequent than CT genotype (P = 0.04). Foetuses with CC genotype VEGF+813 gene have an increased risk for preterm labour. C allele frequency was 90.3 and 76.7% in preterm and control groups, respectively. T allele frequency was 9.7 and 23.3% in preterm and control groups, respectively. C allele was significantly associated with preterm labour (P = 0.02). OR of C and T alleles for preterm labour was 2.8 (CI: 1.1-7.2). In PE group, foetal CC genotype of +813 locus was found in 67.6%, and CT genotype was seen in 29.4%. Only one TT genotype was detected in 2.9% of PE group. There was no association between PE and VEGF gene genotypes and alleles at +813 locus. These results suggest that foetal VEGF gene polymorphism of +813 CC seems to be highly associated with preterm labour, whereas in PE, foetal VEGF gene polymorphism at +813 locus is not related. Especially, C allele was significantly associated with preterm labour. Carriage of the +813C allele of the VEGF gene has been found 2.8 times increased susceptibility to the development of preterm labour in Turkish women and may be an independent risk factor for prematurity. There was no association between PE and VEGF gene genotypes and alleles at +813 locus. We suggest to search for foetal aetiologies or genetic susceptibility in preterm labour, whereas in PE, not foetal, but maternal susceptibility is to be investigated.
我们研究了血管内皮生长因子（VEGF）基因+813 位点多态性对早产和先兆子痫（PE）易感性的影响。我们检查了来自 31 例早产、34 例先兆子痫和 58 例健康足月产婴儿脐带血中 VEGF+813 基因的多态性。使用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析研究了 VEGF+813 基因多态性。在早产组中，胎儿 CC 基因型为 80.6%，CT 基因型为 19.4%。在早产组中未检测到 TT 基因型。VEGF+813 基因的 CC 基因型明显比 CT 基因型更常见（P=0.04）。具有 CC 基因型 VEGF+813 基因的胎儿早产风险增加。C 等位基因频率分别为早产组和对照组的 90.3%和 76.7%，T 等位基因频率分别为早产组和对照组的 9.7%和 23.3%。C 等位基因与早产显著相关（P=0.02）。C 和 T 等位基因对早产的 OR 为 2.8（95%CI：1.1-7.2）。在 PE 组中，胎儿+813 位点的 CC 基因型为 67.6%，CT 基因型为 29.4%。在 2.9%的 PE 组中仅检测到一个 TT 基因型。PE 与 VEGF 基因+813 位点的基因型和等位基因之间没有关联。这些结果表明，胎儿 VEGF 基因+813 CC 多态性与早产高度相关，而在 PE 中，胎儿 VEGF 基因+813 位点多态性与早产无关。特别是，C 等位基因与早产显著相关。在土耳其妇女中，发现 VEGF 基因+813 位 C 等位基因的携带使早产的易感性增加了 2.8 倍，可能是早产的一个独立危险因素。PE 与 VEGF 基因+813 位点的基因型和等位基因之间没有关联。我们建议在早产中寻找胎儿病因或遗传易感性，而在 PE 中，不是胎儿，而是母体的易感性需要研究。
