Unidad de Biotecnología e Ingeniería Genética de Plantas, Cinvestav-Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico.
Planta. 2010 Jan;231(2):397-410. doi: 10.1007/s00425-009-1061-9. Epub 2009 Nov 22.
Root inoculation of tomato (Solanum lycopersicum) plants with a Bacillus subtilis strain BEB-DN (BsDN) isolated from the rhizosphere of cultivated potato plants was able to promote growth and to generate an induced systemic resistance (ISR) response against virus-free Bemisia tabaci. Growth promotion was evident 3 weeks after inoculation. No changes in oviposition density, preference and nymphal number in the early stages of B. tabaci development were observed between BsDN-treated plants and control plants inoculated with a non-growth promoting Bs strain (PY-79), growth medium or water. However, a long-term ISR response was manifested by a significantly reduced number of B. tabaci pupae developing into adults in BsDN-treated plants. The observed resistance response appeared to be a combination of jasmonic acid (JA) dependent and JA-independent responses, since the BsDN-related retardation effect on B. tabaci development was still effective in the highly susceptible spr2 tomato mutants with an impaired capacity for JA biosynthesis. A screening of 244 genes, 169 of which were previously obtained from subtractive-suppressive-hybridization libraries generated from B. tabaci-infested plants suggested that the BsDN JA-dependent ISR depended on an anti-nutritive effect produced by the simultaneous expression of genes coding principally for proteases and proteinase inhibitors, whereas the JA-independent ISR observed in the spr2 background curiously involved the up-regulation of several photosynthetic genes, key components of the phenyl-propanoid and terpenoid biosynthetic pathways and of the Hsp90 chaperonin, which probably mediated pest resistance response(s), in addition to the down-regulation of pathogenesis and hypersensitive response genes.
番茄（Solanum lycopersicum）植株的根部接种从栽培马铃薯根际分离出的枯草芽孢杆菌（Bacillus subtilis）菌株 BEB-DN（BsDN），能够促进生长并对无病毒烟粉虱（Bemisia tabaci）产生诱导系统抗性（ISR）反应。接种后 3 周即可明显促进生长。在烟粉虱发育的早期阶段，BsDN 处理植物与接种非生长促进的 Bs 菌株（PY-79）、生长培养基或水的对照植物之间，未观察到产卵密度、偏好性和若虫数量的变化。然而，BsDN 处理植物中烟粉虱蛹发育成成虫的数量显著减少，表现出长期的 ISR 反应。观察到的抗性反应似乎是茉莉酸（JA）依赖和 JA 不依赖反应的组合，因为 BsDN 对烟粉虱发育的延迟效应在 JA 生物合成能力受损的高敏感 spr2 番茄突变体中仍然有效。对 244 个基因进行筛选，其中 169 个基因先前从受烟粉虱侵害的植物中产生的消减抑制杂交文库中获得，表明 BsDN JA 依赖的 ISR 依赖于同时表达主要编码蛋白酶和蛋白酶抑制剂的基因产生的抗营养作用，而在 spr2 背景中观察到的 JA 不依赖的 ISR 则涉及几个光合作用基因的上调，这些基因是苯丙烷和萜烯生物合成途径以及 Hsp90 伴侣蛋白的关键组成部分，可能介导了抗虫反应，此外还下调了发病机制和过敏反应基因。
