Liu Yan-Ming, Jia Yu-Xiu, Tian Wei
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, PR China.
J Sep Sci. 2008 Dec;31(21):3765-71. doi: 10.1002/jssc.200800373.
A novel and simple method is presented for the determination of norfloxacin, ciprofloxacin, and ofloxacin by capillary electrophoresis with chemiluminescence detection. This method is based on the enhancing effect of quinolones on the chemiluminescence reaction of the Ce(SO(4))(2)-Ru(bpy)(3)(2+)-HNO(3) system. Three quinolones were successfully separated and detected under optimum conditions. The obtained detection limits were 2.3x10(-7) mol/L, 5.2x10(-8) mol/L, and 7.8x10(-8) mol/L for ciprofloxacin, norfloxacin, and ofloxacin, respectively. The RSD of migration time and peak area were less than 1.8 and 3.8% (n = 5), respectively. The applicability of the proposed method was illustrated in the determination of ofloxacin in eye drops and of norfloxacin in human urine samples, and the monitoring of pharmacokinetics for norfloxacin.
提出了一种新颖且简单的方法，用于通过毛细管电泳-化学发光检测法测定诺氟沙星、环丙沙星和氧氟沙星。该方法基于喹诺酮类药物对Ce(SO(4))(2)-Ru(bpy)(3)(2+)-HNO(3)体系化学发光反应的增强作用。在最佳条件下成功分离并检测了三种喹诺酮类药物。环丙沙星、诺氟沙星和氧氟沙星的检测限分别为2.3×10(-7) mol/L、5.2×10(-8) mol/L和7.8×10(-8) mol/L。迁移时间和峰面积的相对标准偏差分别小于1.8%和3.8%（n = 5）。该方法的适用性通过测定滴眼液中的氧氟沙星、人尿样中的诺氟沙星以及监测诺氟沙星的药代动力学得以说明。
