Villarino Nicolas, Cox Sherry, Yarbrough Jason, Martín-Jiménez Tomás
Department of Comparative Medicine, University of Tennessee, College of Veterinary Medicine, Knoxville, TN 37996, USA.
Biomed Chromatogr. 2010 Aug;24(8):908-13. doi: 10.1002/bmc.1385.
Carboplatin is an antineoplastic drug administered to treat different tumoral conditions in canine oncology. The objective of this study was to validate a high-performance chromatographic (HPLC) method which could be applied in canine pharmacokinetic studies. Following ultrafiltration using a Centrifree device, standards, quality controls and plasma samples were separated by isocratic reversed-phase HPLC on an Inertsil ODS-2 (250 x 4.6 mm i.d.) analytical column and quantified using UV detection at 220 nm. The mobile phase was potassium phosphate (pH 4.5), with a flow-rate of 1.0 mL/min. The procedure produced a linear curve (r(2) > 0.999) over the concentration range 1-200 microg/mL. The lower limit of quantification was 1 microg/mL. The intra-assay and inter-assay precision was approximately 90%. The overall recovery was approximately 90%. The method was illustrated with a preliminary pharmacokinetic analysis on nine dogs treated with carboplatin at our hospital. Carboplatin disposition followed a monocompartmental structure in dogs and was characterized by a short half-life (50 min).
卡铂是一种用于治疗犬类肿瘤学中不同肿瘤病症的抗肿瘤药物。本研究的目的是验证一种可应用于犬类药代动力学研究的高效色谱法（HPLC）。使用Centrifree装置进行超滤后，标准品、质量控制品和血浆样品在Inertsil ODS-2（250×4.6 mm内径）分析柱上通过等度反相HPLC分离，并使用220 nm紫外检测进行定量。流动相为磷酸钾（pH 4.5），流速为1.0 mL/min。该方法在1-200μg/mL的浓度范围内产生了线性曲线（r(2)>0.999）。定量下限为1μg/mL。批内和批间精密度约为90%。总回收率约为90%。通过对我院接受卡铂治疗的9只犬进行初步药代动力学分析对该方法进行了说明。卡铂在犬体内的处置遵循单室结构，其特征为半衰期短（50分钟）。
