利用量子点探针的光物理性质进行特定核酸检测。

Specific nucleic acid detection using photophysical properties of quantum dot probes.

机构信息

Diagnostics, Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam City, Gyeonggi-do, Korea, 443-270.

出版信息

Anal Chem. 2010 Feb 1;82(3):886-91. doi: 10.1021/ac902039n.

DOI:10.1021/ac902039n

PMID:20041684

Abstract

In the present study, we report a novel separation-free method to detect and quantify avian influenza virus A (H5N1) nucleic acid without amplification, based on the alteration of photophysical parameters of quantum dot (QD) probes after hybridization with specific complementary target DNA. The target DNA was quantified in a custom-made portable device by simultaneously measuring lifetime and quenching of the QD probes. QD probes (25-mer) showed a 30% lifetime reduction and 40% fluorescence quenching when hybridized with complementary 25-mer target DNA. In comparison with a conventional QD-based assay, this assay provides a simple quantitation of nucleic acids with a single labeling step.

摘要

在本研究中，我们报告了一种新颖的无需分离即可检测和定量禽流感病毒 A（H5N1）核酸的方法，该方法基于量子点（QD）探针与特定互补目标 DNA 杂交后光物理参数的变化。通过同时测量 QD 探针的寿命和猝灭，在定制的便携式设备中定量检测目标 DNA。与传统的基于 QD 的测定方法相比，该测定方法仅需一个标记步骤，即可提供简单的核酸定量。QD 探针（25 个碱基）与互补的 25 个碱基目标 DNA 杂交时，寿命降低了 30%，荧光猝灭了 40%。