Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
J Nat Prod. 2010 Mar 26;73(3):441-6. doi: 10.1021/np900685z.
Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A-E (1-5). The structures were established through 2D-NMR experiments and mass spectrometry, as well as the synthesis of dapdiamide A (1). Transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. The dapdiamide family's promiscuous biosynthetic pathway contains two unconventional amide ligases that are predicted to couple its constituent monomers.
从 Pantoea agglomerans 菌株 CU0119 构建基因组 DNA 文库，并针对植物病原体 Erwinia amylovora 进行筛选，得到了一种新的抗生素家族 dapdiamides A-E（1-5）。通过 2D-NMR 实验和质谱以及 dapdiamide A（1）的合成确定了这些结构。活性 cosmid 的转座子诱变允许鉴定生物合成基因簇。dapdiamide 家族的混杂生物合成途径包含两个非常规的酰胺连接酶，据预测它们可以连接其组成单体。
